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Method for detecting pulmonary tuberculosis diseases based on human plasma autofluorescence spectra

An autofluorescence and detection method technology, applied in the field of medical disease diagnosis, can solve the problems of long time to obtain diagnostic results, low diagnostic sensitivity, and expensive inspection costs, and achieve high diagnostic sensitivity and accuracy, high detection safety, and detection fast effect

Inactive Publication Date: 2020-05-19
SICHUAN UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of low diagnostic sensitivity, high inspection cost, long time for obtaining diagnostic results and invasive inspection in the current clinical methods for diagnosing pulmonary tuberculosis. its application

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  • Method for detecting pulmonary tuberculosis diseases based on human plasma autofluorescence spectra
  • Method for detecting pulmonary tuberculosis diseases based on human plasma autofluorescence spectra
  • Method for detecting pulmonary tuberculosis diseases based on human plasma autofluorescence spectra

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Embodiment Construction

[0020] The present invention will be further illustrated by specific description below.

[0021] 1. Preparation of human plasma samples

[0022] During the period from 7:00 to 8:00 in the morning, the subject’s overnight fasting venous blood was drawn under aseptic conditions, collected into vacuum blood collection tubes containing EDTA anticoagulant, and then placed in a small centrifuge at a speed of 2500 rpm. Process for 10-15 minutes. Take about 1-2ml of plasma from the centrifuge tube with a pipette gun, and put it into a sterilized quartz cuvette.

[0023] 2. Fluorescence spectrum detection process

[0024] Put the quartz cuvette containing the plasma sample into the sample cell of the fluorescence spectrophotometer, and operate the fluorescence spectrophotometer to measure the autofluorescence spectrum of the plasma (see attached figure 1 As shown), the measurement parameters used are: excitation wavelength 400nm, fluorescence scanning wavelength range 425-675 nm, sc...

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Abstract

The invention provides a method for diagnosing pulmonary tuberculosis diseases by detecting characteristic peaks of autofluorescence spectra of human plasma and combining a multivariable statistical analysis method and application of the method. The method comprises the following steps: collecting fasting vein blood of a subject, performing centrifugal treatment to separate plasma, measuring the autofluorescence spectrum of the plasma by using a fluorospectro photometer, carrying out second derivative treatment on the measured fluorescence spectrum, preliminarily screening pulmonary tuberculosis patients according to characteristic peaks of the fluorescence spectrum; and processing the fluorescence spectrum data by using a multivariable statistical analysis method to obtain score data corresponding to pulmonary tuberculosis disease characteristics, and comparing the score data with discrimination parameters or discrimination equations in a self-built database to diagnose whether the subject suffers from pulmonary tuberculosis diseases. The detection method provided by the invention is convenient in sampling, rapid in detection, high in diagnosis sensitivity, specificity and accuracy and low in cost, can provide a novel detection method for the diagnosis of pulmonary tuberculosis diseases, and has important social and economic values and clinical application values.

Description

technical field [0001] The invention belongs to the field of medical disease diagnosis. Specifically, it is a method for diagnosing pulmonary tuberculosis and its application by detecting the characteristic peaks of the autofluorescence spectrum of human plasma, combined with a multivariate statistical analysis method. Background technique [0002] Tuberculosis is mainly transmitted through the air, and can be transmitted through the droplets of people coughing and sneezing. It is the number one killer of infectious diseases in the world and one of the top ten causes of death in the world. According to the World Health Organization, there are millions of new cases of tuberculosis every year. In 2017 alone, about 10 million people were infected with tuberculosis, and 1.3 million people died of tuberculosis. The World Health Organization also designated March 24 as "World Tuberculosis Day". As one of the countries with a high burden of tuberculosis in the world, China ranks ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 朱建华王悦铮陈雪融
Owner SICHUAN UNIV
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