Method for inhibiting tumor cells from growing by radiofrequency ablation

A technology for radiofrequency ablation and tumor application in the field of expanding the range of tumor ablation to achieve the effect of improving physical properties

Pending Publication Date: 2020-05-22
SHANGHAI UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems existing in the prior art and provide a method for expanding the range of tumor ablation by radiofrequency ablation, which

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  • Method for inhibiting tumor cells from growing by radiofrequency ablation
  • Method for inhibiting tumor cells from growing by radiofrequency ablation
  • Method for inhibiting tumor cells from growing by radiofrequency ablation

Examples

Experimental program
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Embodiment 1

[0025] Embodiment one: see figure 1 with figure 2

[0026] HepG2 liver cancer cells were cultured in cell culture medium (Dulbecco modified Eagle medium, DMEM) containing 10% fetal bovine serum and 1% penicillin / streptomycin (PS), and the incubator environment was CO 2 Concentration 5% and temperature 37°C. Before use, cells were passed through 0.25% trypsin-EDTA solution for harvesting, and passed the cell counter ( BioTech, Shanghai Ruiyu Biotech.Co., Ltd., Shanghai, China) ensure that the cell concentration in the cell suspension is 2×106cells / ml (overall viability >97%). In addition, COS and CMC powders are added to DMEM respectively to form DMEM solutions containing different concentrations of COS and CMC, called COS DMEM1,10,25 and CMC DMEM1,10,25 (Number subscripts are the concentrations used, ie 1, 10 and 25 mg / mL). Additionally, all solutions were sterilized by passing through 0.22 μm filters and UV light. HepG2 cells were transplanted into 12-well plates a...

Embodiment 2

[0029] Embodiment two: see image 3 .

[0030] In addition to the inhibitory effect of COS and CMC on tumor cells, COS and CMC can also improve the physical properties of biological tissues, that is, electrical conductivity, to promote the outward diffusion of radiofrequency current and expand the therapeutic range of radiofrequency ablation. In this experiment, the liver model was used to measure the conductivity. The initial conductivity measurement experiment of the liver model without solution was used as the control group, and the experimental group was injected with COS DW or CMC DW Measurement of the posterior liver conductivity. COS DW or CMC DW Fully inject into the model through the syringe to ensure COS within the measurement range DW or CMC DW Consistent concentration, the whole experiment used three concentrations of COS: 10, 50, 100mg / mL DW and CMC DW The measurement experiment was carried out, and the conductivity measurement device was a four-probe meas...

Embodiment 3

[0032] Embodiment three: see Figure 4 , Figure 5 with Image 6

[0033] In the experiment, a commercial bipolar gold-plated electrode needle (Genetrode, 508, Harvard Apparatus, Holliston, MA, USA) was used to perform radiofrequency ablation on the liver model. Place the two electrode needles at a distance of 5mm and insert them into the model in parallel with a depth of 5mm. Then inject 2mL (total 4mL) of cationic polymer solution at the axial midpoint of the two electrodes of the bipolar electrode needle. In this experiment, they were randomly divided into three groups according to the injected solution, namely, the control group (no solution injection), COS DW100 Group and CMC DW100 groups, and each group performed 4 experiments. After solution injection, an alternating current of 0.06 A was input into the liver model through bipolar electrode needles. When the current input of the tissue is blocked due to high-temperature carbonization, that is, roll-off, the entir...

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Abstract

The invention relates to a method for expanding an ablation range of tumors by radiofrequency ablation. The method includes the following specific steps: (1) dissolving a cationic polymer, and performing filter sterilization and ultraviolet irradiation sterilization; (2) inserting an electrode needle into a target tissue position in a puncture manner; (3) injecting the cationic polymer solution near the electrode needle; (4) performing radiofrequency ablation, and introducing radiofrequency energy into target tissue through the electrode needle to realize tissue heating; (5) after the ablationis completed, taking out the electrode needle, and retaining the cationic polymer solution in the target tissue; and (6) finally, discharging the cationic polymer solution out of bodies through a tissue metabolism and circulation system. The method has the following advantages: (1) the cationic polymer can effectively optimize the tissue environment of the radiofrequency ablation and improve therange of ablation; and (2) the inhibitory effect of the cationic polymer on the tumors can promote the complete ablation of the tumors.

Description

technical field [0001] The invention relates to a method for expanding the range of tumor ablation, in particular to expanding the range of tumor ablation by cooperating with cationic polymers for radiofrequency ablation. Background technique [0002] Radiofrequency ablation is a technology that uses radiofrequency current to generate resistive heat in biological tissues, and then ablate diseased tissues. Since the 20th century, as a minimally invasive tumor treatment technique, radiofrequency ablation has been widely used in clinical tumor treatment due to its advantages of less trauma, less pain for patients, and short postoperative recovery time. The whole radiofrequency ablation system is mainly composed of a radiofrequency power supply, an electrode needle and a grounding plate. The RF power supply can provide a 375-500kHz alternating current to form an alternating electric field between the electrode needle and the grounding plate, and then under the action of the ele...

Claims

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Application Information

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IPC IPC(8): A61B18/12A61B18/14C12N5/09G01N27/04
CPCA61B18/12A61B18/14A61B2018/00529A61B2018/00577A61B2018/1425C12N5/0693C12N2500/84G01N27/041
Inventor 方正张兵章文俊高敏
Owner SHANGHAI UNIV
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