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Method for detecting PhIP content in frying oil

A frying oil and content technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of health hazards, high PhIP, etc., and achieve the effect of high precision, fast speed and wide application prospects

Pending Publication Date: 2020-05-26
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under the conditions of high-content oil and high-temperature processing of fried food, food components will produce harmful substances such as heterocyclic amines and acrylamide through complex chemical reactions, and these harmful substances will migrate into the oil. When the oil is repeatedly fried, the content of PhIP may become higher and higher, which will cause potential harm to people's health

Method used

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  • Method for detecting PhIP content in frying oil
  • Method for detecting PhIP content in frying oil
  • Method for detecting PhIP content in frying oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Extraction of PhIP:

[0034] Accurately weigh 3.00g of soybean oil into a Hach tube, add 10μL of PhIP standard solution with a concentration of 5mg / L, 10mg / L, and 25mg / L, respectively, and add levels of 50μg / L, 100μg / L, and 250μg / L , Add 7 mL of n-butanol and vortex mix for 5 min.

[0035] The next step is to perform propanesulfonic acid solid-phase extraction column extraction: first activate with 8mL methanol, adjust the flow rate to 1-2d / s, and when it is about to drip dry, add 8mL0.1mol / L hydrochloric acid: water (v / v=1 : 1) solution and 6mL n-butanol solution. Then, the n-butanol sample solution was loaded, and the prepared sample solution and 5mL rinse solution were added to the column, and the flow rate was adjusted to 2-3s per drop to ensure that the sample solution was adsorbed on the column packing as much as possible. After the sample in the column is dripped clean, rinse the column with 5mL0.01mol / L hydrochloric acid solution and 5mL0.1mol / L hydrochloric a...

Embodiment 2

[0062] Extraction of PhIP:

[0063] Accurately weigh 3.00 g of peanut oil into a Hash tube, add 10 μL of PhIP standard solution with a concentration of 5 mg / L, 10 mg / L, and 25 mg / L, and add levels of 50 μg / L, 100 μg / L, and 250 μg / L, respectively. Add 7 mL of n-butanol and vortex mix for 5 min.

[0064] The next step is to perform propanesulfonic acid solid-phase extraction column extraction: first activate with 8mL methanol, adjust the flow rate to 1-2d / s, and when it is about to drip dry, add 8mL0.1mol / L hydrochloric acid: water (v / v=1 : 1) solution and 6mL n-butanol solution. Then, the n-butanol sample solution was loaded, and the prepared sample solution and 5mL rinse solution were added to the column, and the flow rate was adjusted to 2-3s per drop to ensure that the sample solution was adsorbed on the column packing as much as possible. After the sample in the column is dripped clean, rinse the column with 5mL0.01mol / L hydrochloric acid solution and 5mL0.1mol / L hydrochl...

Embodiment 3

[0091] Extraction of PhIP:

[0092] Accurately weigh 3.00g of palm oil into a hash tube, add 10μL of PhIP standard solution with a concentration of 5mg / L, 10mg / L, and 25mg / L, and add levels of 50μg / L, 100μg / L, and 250μg / L respectively , Add 7 mL of n-butanol and vortex mix for 5 min.

[0093] The next step is to perform propanesulfonic acid solid-phase extraction column extraction: first activate with 8mL methanol, adjust the flow rate to 1-2d / s, and when it is about to drip dry, add 8mL0.1mol / L hydrochloric acid: water (v / v=1 : 1) solution and 6mL n-butanol solution. Then, the n-butanol sample solution was loaded, and the prepared sample solution and 5mL rinse solution were added to the column, and the flow rate was adjusted to 2-3s per drop to ensure that the sample solution was adsorbed on the column packing as much as possible. After the sample in the column is dripped clean, rinse the column with 5mL0.01mol / L hydrochloric acid solution and 5mL0.1mol / L hydrochloric acid:...

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Abstract

The invention discloses a method for detecting PhIP content in frying oil. The method comprises the following steps: pretreating a sample and detecting by adopting a liquid chromatograph-mass spectrometer. According to the method for detecting the PhIP content, liquid chromatography conditions for detecting PhIP in the frying oil and optimized mass spectrum parameters are provided, the recovery rate is good, and the method for extracting PhIP in the frying oil is easy to operate, high in precision and high in speed and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of food processing control, and specifically relates to a technique for detecting PhIP content in frying oil. Background technique [0002] PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) full name 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine, belongs to heterocycle A type of amine. Ingestion of human body can induce various cancers, which is extremely harmful to human health. Heterocyclic amines can be divided into two types according to the structure, one is: aminoimidazoazarenes (aminoimidazoazarenes) or thermally polymerized heterocyclic amines (thermic HAAs), also known as IQ type (imidazoquinoline or imidazoquinoxaline type) heterocyclic amines; Yes: carbolines or pyrolytic heterocyclic amines (pyrolytic HAAs), also known as nonimidazoquinoline or non-imidazoquinoxaline type heterocyclic amines, in which aminoimidazoquinoxaline can be divided into three categories: pyridine imidazopyridine derivati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张燕王晓敏刘冰王硕
Owner TIANJIN UNIV OF SCI & TECH
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