Kit for detecting echinococcosis

A kit and technology for hydatid disease, applied in the field of kits, can solve the problems of low detection efficiency, missed diagnosis, difficulty in typing, misdiagnosis, etc., and achieve the effects of high detection accuracy, high detection efficiency and simple operation.

Pending Publication Date: 2020-06-05
NANJING YIKE POPULATION HEALTH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a diagnostic kit for detecting echinococcosis, which solves the problem of misdiagnosis, misdiagnosis and classification of small lesions and atypical lesions in the current detection technology of echinococcosis. The accuracy of echinococcosis detection results cannot be guaranteed, and the detection efficiency is low

Method used

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  • Kit for detecting echinococcosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] S1. Take 3 g of parasite tissue to be inspected or infected lesions of human body or animal into a test tube containing 2 ml of preservation solution. After mixing thoroughly, centrifuge at 2500 rpm for 10 minutes, extract the whole genome DNA of the sample and place it in the PCR reaction. reserve in the tube;

[0029] S2. Add 2 parts of upstream primer Eg-F, 2 parts of downstream primer Eg-R, 3 parts of dNTp mixture, 1 part of DNA polymerase, 1 part of Taq enzyme, and trimethylol into the PCR reaction tube containing the whole genome DNA of the sample in S1. 2 parts of aminomethane hydrochloric acid solution and 6 parts of sterile double distilled water, fully mixed;

[0030] S3. Place the PCR reaction tube in S2 in a PCR machine for PCR amplification, spot the amplified PCR product on a 3% agarose gel for electrophoresis, and observe the electrophoresis results, wherein the reaction condition of PCR is 94°C Pre-denaturation for 3 minutes, denaturation at 94°C for 35...

Embodiment 2

[0033] S1. Take 3 g of parasite tissue to be inspected or infected lesions of human body or animal into a test tube containing 2 ml of preservation solution. After mixing thoroughly, centrifuge at 2500 rpm for 10 minutes, extract the whole genome DNA of the sample and place it in the PCR reaction. reserve in the tube;

[0034] S2. Add 4 parts of upstream primer Eg-F, 4 parts of downstream primer Eg-R, 5 parts of dNTp mixture, 3 parts of DNA polymerase, 3 parts of Taq enzyme, and trimethylol into the PCR reaction tube containing the whole genome DNA of the sample in S1. 4 parts of aminomethane hydrochloric acid solution and 8 parts of sterile double distilled water, fully mixed;

[0035] S3. Place the PCR reaction tube in S2 in a PCR machine for PCR amplification, spot the amplified PCR product on a 3% agarose gel for electrophoresis, and observe the electrophoresis results, wherein the reaction condition of PCR is 94°C Pre-denaturation for 3 minutes, denaturation at 94°C for ...

Embodiment 3

[0038] S1. Take 3 g of parasite tissue to be inspected or infected lesions of human and animals into a test tube containing 2 ml of preservation solution. After mixing thoroughly, centrifuge at 2500 rpm for 10 minutes, extract the whole genome DNA of the sample and place it in the PCR reaction. reserve in the tube;

[0039]S2. Add 3 parts of upstream primer Eg-F, 3 parts of downstream primer Eg-R, 4 parts of dNTp mixture, 2 parts of DNA polymerase, 2 parts of Taq enzyme, and trimethylol into the PCR reaction tube containing the whole genome DNA of the sample in S1. 3 parts of aminomethane hydrochloric acid solution and 7 parts of sterile double distilled water, fully mixed;

[0040] S3. Place the PCR reaction tube in S2 in a PCR machine for PCR amplification, spot the amplified PCR product on a 3% agarose gel for electrophoresis, and observe the electrophoresis results, wherein the reaction condition of PCR is 94°C Pre-denaturation for 3 minutes, denaturation at 94°C for 35s,...

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Abstract

The invention relates to the technical field of kits, and discloses a kit for detecting echinococcosis. The kit comprises the following raw materials in parts by weight: 2-4 parts of upstream primer Eg-F, 2-4 parts of downstream primer Eg-R, 3-5 parts of dNTp mixture, 1-3 parts of DNA polymerase, 1-3 parts of Taq enzyme, 2-4 parts of trimethylolaminomethane hydrochloride solution, and 6-8 parts ofsterile double-distilled water. According to the kit, 3 g of hydatid body tissue or human or animal infection foci to be tested are placed in a test tube containing 2 ml of a preservation solution, and after thorough mixing, centrifugation is performed at 2500 rpm for 10 minutes, and whole genome DNA of the extracted sample is placed in a PCR reaction tube for later use. The kit has high accuracyfor detection results of echinococcosis, is simple for a user to operate, is not prone to misdiagnosis, missed diagnosis and difficult classification of small lesions and atypical lesions, and further has high detection efficiency for echinococcosis and can timely and accurately detect echinococcosis.

Description

technical field [0001] The invention relates to the technical field of kits, in particular to a kit for detecting and diagnosing echinococcosis. Background technique [0002] Hydatid disease, also known as echinococcosis, is a disease caused by the larvae of Echinococcus granulosus infecting the human body. The egg becomes the intermediate host and suffers from echinococcosis. The adult Echinococcus granulosus parasitizes in the small intestine of the dog. It is the smallest type of tapeworm in the family. The egg is round or oval, with a diameter of about 30-40um. It is hexahelcaria and has strong resistance to the external environment. [0003] At present, the diagnosis of echinococcosis mainly relies on B-ultrasound, CT and other imaging techniques and pathological examinations. Due to the limitations of the method itself, it is easy to cause misdiagnosis, missed diagnosis and difficult classification of small lesions and atypical lesions, especially for echinococcosis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686
CPCC12Q1/6888C12Q1/686C12Q2565/125
Inventor 戴俊程江玥何元林
Owner NANJING YIKE POPULATION HEALTH RES INST CO LTD
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