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Stenotrophomonas maltophilia GYH and application thereof in degradation of chlorinated hydrocarbon pollutants

A Stenotrophomonas and maltophilia technology, which is applied to Stenotrophomonas maltophilia GYH and its application field in degrading chlorinated hydrocarbon pollutants, can solve problems such as no high-efficiency degradation found, and achieve high-efficiency degradation effect of ability

Active Publication Date: 2020-06-09
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, it is necessary to study the degradation of chloroform in the environment for human health. Through literature search, there are some reports about the biodegradation of polycyclic aromatic hydrocarbons, benzopyrene, and cyclotrimethylenetrinitramine by Stenotrophomonas maltophilia, but no relevant reports have been found. A report on efficient degradation of Stenotrophomonas maltophilia using chloroform as the sole carbon source

Method used

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  • Stenotrophomonas maltophilia GYH and application thereof in degradation of chlorinated hydrocarbon pollutants
  • Stenotrophomonas maltophilia GYH and application thereof in degradation of chlorinated hydrocarbon pollutants
  • Stenotrophomonas maltophilia GYH and application thereof in degradation of chlorinated hydrocarbon pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Isolation, purification and identification of Stenotrophomonas maltophilia GYH

[0037] 1. Separation and purification of Stenotrophomonas maltophilia GYH

[0038] Stenotrophomonas maltophilia GYH is a Gram-negative bacterium domesticated and isolated from activated sludge. The specific steps are as follows:

[0039] Add 50mL of inorganic salt medium to a 300mL shake flask, and add 10mL of activated sludge and 8.9mg / L of chloroform for enrichment culture. When the concentration of chloroform is 50% of the initial concentration, take out 5mL of enrichment solution Add chloroform with a final concentration of 8.9 mg / L to 50 mL of fresh inorganic salt medium, repeat the above enrichment process 5 times, then apply LB solid medium by gradient dilution of the last enrichment solution, and select a single colony to inoculate and isolate Plate streak purification ( figure 1 ), add the bacterium colony that growth obtains to inorganic salt culture medium, and add f...

Embodiment 2

[0056] Example 2 Obtaining of Stenotrophomonas maltophilia GYH Resting Cells

[0057] 1. Incline cultivation:

[0058] Inoculate Stenotrophomonas maltophilia CCTCC NO: M 20191025 into LB liquid medium, culture at 30°C, 160rpm for 2 days, then streak the activated bacteria on the LB solid plate in a 30°C incubator for 24 hours, and take a single Bacterial colonies continue to be streaked on the plate to detect the purity of the bacteria, and the LB test tubes are stored on the slant (4°C) routinely.

[0059] 2. Expand training

[0060] Inoculate the slant bacteria in step 1 into LB liquid medium, culture at 30°C, 160rpm for 12 hours, obtain the expanded culture medium, centrifuge, collect the wet bacteria, wash with inorganic salt culture solution, and obtain Stenotrophomonas maltophilia GYH resting cells.

Embodiment 3

[0061] Example 3: Detection of the degradation performance of Stenotrophomonas maltophilia GYH on different concentrations of chloroform

[0062] The inorganic salt culture medium was divided into 330mL shake flasks each with a volume of 50mL, and sterilized at 110°C for 40min. After the sterilization, it was placed at room temperature for 2 days to confirm that there was no growth of miscellaneous bacteria. Add the Stenotrophomonas maltophilia GYH quiescent cells that the final concentration reaches 80mg / L (recorded in dry cell weight) embodiment 2 method, then add chloroform as the sole carbon source to make the final concentration respectively 2.98,5.93 , 8.93, 14.85, 20.97, 29.8mg / L, after the shaker flask was sealed, cultured on a shaker at 30°C and 160rpm, and a blank control without adding bacteria was made. Regularly measure the concentration of chloroform remaining in the shake flask, and regularly measure the generation of chloride ions in the solution, draw the tim...

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Abstract

The invention discloses stenotrophomonas maltophilia GYH and application thereof in degradation of chlorinated hydrocarbon pollutants. The application comprises the following steps: adding resting cells obtained by expanding culture of stenotrophomonas maltophilia GYH into an inorganic culture medium with a pH value of 5-8, then adding chlorinated hydrocarbon pollutants, and conducting culturing under the conditions of a temperature of 20-35 DEG C and a rotation speed of 140-180 rpm to realize degradation of the pollutants. The stenotrophomonas maltophilia GYH disclosed by the invention can beused for removing chlorinated hydrocarbon pollutants with concentrations in a range of 2.9 mg / L to 8.93 mg / L. Therefore, the stenotrophomonas maltophilia has efficient degradation capability on industrial similar pollutants, and can bear pollutants with a relatively high concentration.

Description

[0001] (1) Technical field [0002] The invention relates to Stenotrophomonas maltophilia GYH and its application in degrading pollutants such as chloroform. [0003] (2) Background technology [0004] Chlorinated hydrocarbons are halogenated hydrocarbons formed by replacing hydrogen atoms in hydrocarbon molecules with chlorine atoms. Halogenated hydrocarbons are divided into fluorohydrocarbons, chlorinated hydrocarbons, bromohydrocarbons and iodohydrocarbons according to the substitution of halogen; they can also be divided into monohalogenated hydrocarbons, dihalogenated hydrocarbons and polyhalogenated hydrocarbons according to the number of halogen atoms in the molecule. Hydrocarbons. Lower hydrocarbons are gases or liquids, and higher hydrocarbons are solids. Most halogenated hydrocarbons are insoluble in water or have little solubility in water, but can be dissolved in organic solvents. Most halogenated hydrocarbons have a special smell. Halogenated hydrocarbons are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02A62D101/22C12R1/01
CPCC12N1/20A62D3/02A62D2101/22C12R2001/01C12N1/205C02F2101/32C02F2101/36C02F3/341
Inventor 成卓韦陈东之王家德於建明官艳红
Owner ZHEJIANG UNIV OF TECH
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