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A method for reclaiming and extracting SOD from coniferous cherry pomace

A technology of acerola cherry and crude extract, which is applied in the direction of biochemical equipment and methods, enzymes, oxidoreductase, etc., and can solve the problems of ineffective utilization of acerola cherry pomace, low utilization rate of acerola cherry, SOD waste, etc. problems, to achieve high specific activity, low equipment requirements and high extraction rate

Active Publication Date: 2022-06-24
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the low utilization rate of the existing acerola cherries, most of them only extract the juice after simple pressing and use the juice to make products, and the pressed acerola cherry pomace cannot be effectively utilized, resulting in a large amount of nutrients, especially in the In the process of juicing, there is a large amount of SOD waste in the dregs. The invention provides a method for recovering and extracting SOD from coniferous cherry dregs.

Method used

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  • A method for reclaiming and extracting SOD from coniferous cherry pomace
  • A method for reclaiming and extracting SOD from coniferous cherry pomace

Examples

Experimental program
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Effect test

Embodiment 1

[0071] A method for recovering and extracting SOD from acerola slag, the steps are as follows:

[0072] S1, get the acerola slag of 1kg as raw material, carry out sieving to remove impurities, clean the acerola slag and be broken into pieces of ≤ 2mm with a beater, to obtain broken powder;

[0073] S2, adding the shredded powder and cellulase described in step S1 to the water of 2 times the mass of the shredded powder at a mass ratio of 1g:600U, so that the water is completely covered by the shredded powder, and the frequency is 20kHz and the power is 100W. Ultrasonic crushing for 10min, using a 160 mesh screen to filter to remove the filter residue to obtain a filtrate;

[0074] S3, filter the filtrate described in step S2 with a filter membrane with a molecular weight cut-off of 50,000 Da to obtain a first-level filtrate and a first-level retentate, and then use a filter membrane with a molecular weight cut-off of 30,000 Da to filter the first-level filtrate to obtain a seco...

Embodiment 2

[0081] A method for recovering and extracting SOD from acerola slag, the steps are as follows:

[0082] S1, get the acerola slag of 1kg as raw material, carry out sieving to remove impurities, clean the acerola slag and be broken into pieces of ≤ 2mm with a beater, to obtain broken powder;

[0083] S2, adding the shredded powder and cellulase described in step S1 to the water of 3 times the mass of the shredded powder at a mass ratio of 1g:900U, so that the water is completely covered by the shredded powder, and the frequency is 20kHz and the power is 125W. Ultrasonic crushing for 14min, using a 160-mesh screen to filter to remove the filter residue to obtain a filtrate;

[0084] S3, filter the filtrate described in step S2 using a filter membrane with a molecular weight cut-off of 55,000 Da to obtain a first-level filtrate and a first-level retentate, and then use a filter membrane with a molecular weight cut-off of 20,000 Da to filter the first-level filtrate to obtain a sec...

Embodiment 3

[0089] A method for recovering and extracting SOD from acerola slag, the steps are as follows:

[0090] S1, get the acerola slag of 1kg as raw material, carry out sieving to remove impurities, clean the acerola slag and be broken into pieces of ≤ 2mm with a beater, to obtain broken powder;

[0091] S2, adding the shredded powder and cellulase described in step S1 to the water of 2 times the mass of the shredded powder at a mass ratio of 1g:700U, so that the water is completely covered by the shredded powder, and the frequency is 10kHz and the power is 50W. Ultrasonic crushing for 10min, using a 160 mesh screen to filter to remove the filter residue to obtain a filtrate;

[0092] S3, filter the filtrate described in step S2 with a filter membrane with a molecular weight cut-off of 50,000 Da to obtain a first-level filtrate and a first-level retentate, and then use a filter membrane with a molecular weight cut-off of 30,000 Da to filter the first-level filtrate to obtain a second-...

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Abstract

The invention relates to the field of recycling of agricultural and sideline products, in particular to a method for recovering and extracting SOD from coniferous cherry residue; the method comprises the steps of: using coniferous cherry residue as a raw material, preprocessing and crushing to obtain fine powder; ultrasonic crushing and leaching , filtering to remove the filter residue to obtain the filtrate; graded filtration and cut-off combination to obtain the crude extract; refining the SOD in the crude extract and then freeze-drying the refined SOD solution to obtain the SOD powder. The invention can realize efficient and rapid recovery and extraction of SOD in coniferous cherry pomace; the method is simple and efficient, and requires less equipment; the extraction rate of SOD is high, and the extracted SOD has higher purity and higher specific activity.

Description

technical field [0001] The invention relates to the field of recycling and utilization of agricultural and sideline products, in particular to a method for recycling and extracting SOD from acerola slag. Background technique [0002] Acerola cherries (Malpighia emarginata D.C.) are the fruits of the genus Syringa genus in the family Sagittarius, which are rich in a variety of vitamins and minerals. In particular, it contains a unique anti-aging factor (SOD), also known as superoxide dismutase, which is an important component of the antioxidant enzyme system in the organism. Medical research shows that SOD has a certain curative effect on various diseases, especially in the treatment of rheumatoid arthritis, lupus erythematosus and dermatomyositis, and also has good effects in radiation protection, anti-aging and anti-tumor. Performance. [0003] Most plants and microorganisms contain this substance. According to the different metal prosthetic groups, it is mainly divided i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 许明峰胡蒋宁孙长玲单诗慧郭晓璐张铁涛王卉李玲玲蒋思伟
Owner DALIAN POLYTECHNIC UNIVERSITY