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Method for recovering and extracting SOD from conifer cherry residues

A technology of acerola and crude extract, applied in biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problem of low utilization rate of acerola, ineffective utilization of acerola slag, waste of SOD, etc. problem, to achieve the effect of high specific activity, low equipment requirements, and high extraction rate

Active Publication Date: 2020-06-09
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the low utilization rate of the existing acerola cherries, most of them only extract the juice after simple pressing and use the juice to make products, and the pressed acerola cherry pomace cannot be effectively utilized, resulting in a large amount of nutrients, especially in the In the process of juicing, there is a large amount of SOD waste in the dregs. The invention provides a method for recovering and extracting SOD from coniferous cherry dregs.

Method used

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  • Method for recovering and extracting SOD from conifer cherry residues
  • Method for recovering and extracting SOD from conifer cherry residues

Examples

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Effect test

Embodiment 1

[0071] A kind of coniferous cherry pomace reclaims and extracts the method for SOD, and the steps are as follows:

[0072] S1. Take 1 kg of coniferous cherry residue as a raw material, carry out sub-sieving to remove impurities, clean the coniferous cherry residue and crush it with a beater into fragments of ≤ 2mm to obtain crushed powder;

[0073] S2. Add the crushed powder and cellulase described in step S1 to water twice the mass of the crushed powder at a mass ratio of 1g:600U, so that the water is completely submerged in the crushed powder, and the frequency is 20kHz, and the power is 100W. Ultrasonic crushing for 10 minutes, using a 160-mesh sieve to filter and remove the filter residue to obtain the filtrate;

[0074] S3. Use a filter membrane with a molecular weight cut-off of 50000Da to filter the filtrate described in step S2 to obtain a primary filtrate and a primary retentate, and then use a filter membrane with a molecular weight cut-off of 30000Da to filter the p...

Embodiment 2

[0081] A kind of coniferous cherry pomace reclaims and extracts the method for SOD, and the steps are as follows:

[0082] S1. Take 1 kg of coniferous cherry residue as a raw material, carry out sub-sieving to remove impurities, clean the coniferous cherry residue and crush it with a beater into fragments of ≤ 2mm to obtain crushed powder;

[0083] S2. Add the crushed powder and cellulase described in step S1 to water with a mass 3 times the mass of the crushed powder at a mass ratio of 1g:900U, so that the water is completely submerged in the crushed powder, and the frequency is 20kHz, and the power is 125W. Ultrasonic crushing for 14 minutes, using a 160-mesh sieve to filter and remove the filter residue to obtain the filtrate;

[0084] S3. Use a filter membrane with a molecular weight cut-off of 55000Da to filter the filtrate described in step S2 to obtain a primary filtrate and a primary retentate, and then use a filter membrane with a molecular weight cut-off of 20000Da t...

Embodiment 3

[0089] A kind of coniferous cherry pomace reclaims and extracts the method for SOD, and the steps are as follows:

[0090] S1. Take 1 kg of coniferous cherry residue as a raw material, carry out sub-sieving to remove impurities, clean the coniferous cherry residue and crush it with a beater into fragments of ≤ 2mm to obtain crushed powder;

[0091] S2. Add the crushed powder and cellulase described in step S1 to water twice the mass of the crushed powder at a mass ratio of 1g:700U, so that the water is completely submerged in the crushed powder, and the frequency is 10kHz, and the power is 50W. Ultrasonic crushing for 10 minutes, using a 160-mesh sieve to filter and remove the filter residue to obtain the filtrate;

[0092] S3. Use a filter membrane with a molecular weight cut-off of 50000Da to filter the filtrate described in step S2 to obtain a primary filtrate and a primary retentate, and then use a filter membrane with a molecular weight cut-off of 30000Da to filter the prim...

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Abstract

The invention relates to the field of recycle of agricultural and sideline products, and in particular to a method for recovering and extracting SOD from conifer cherry residues. The method comprisesthe following steps: pre-treating and crushing conifer cherry residues serving as raw materials to obtain crushed powder; carrying out ultrasonic crushing and leaching, carrying out filtering, and removing filter residues to obtain a filtrate; carrying out grading filtration and interception in cooperation to obtain a crude extract; and refining SOD in the crude extract, and freeze-drying the refined SOD solution to obtain SOD powder. The method disclosed by the invention can be used for efficiently and rapidly recovering and extracting SOD in conifer cherry residues; the method is simple andefficient, and has low requirements on equipment; and the extraction rate of SOD is high, and the extracted SOD has a higher purity and high specific activity.

Description

technical field [0001] The invention relates to the field of recycling of agricultural and sideline products, in particular to a method for recycling and extracting SOD from coniferous cherry pomace. Background technique [0002] Acerola cherry (Malpighia emarginata D.C.) is the fruit of Acerola genus Acerola in the Aceraceae family, which is rich in various vitamins and minerals. In particular, it contains a unique anti-aging factor (SOD), also known as superoxide dismutase, which is an important component of the antioxidant enzyme system in organisms. Medical research shows that: SOD has a certain curative effect on various diseases, especially in the treatment of rheumatoid arthritis, lupus erythematosus and dermatomyositis, etc. It also has good effects in radiation protection, anti-aging and anti-tumor. Performance. [0003] Most plants and microorganisms contain this substance. According to the different metal prosthetic groups, it is mainly divided into three types:...

Claims

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Application Information

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IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 许明峰胡蒋宁孙长玲单诗慧郭晓璐张铁涛王卉李玲玲蒋思伟
Owner DALIAN POLYTECHNIC UNIVERSITY
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