Microbial identification and analysis system and device based on next-generation high-throughput sequencing

Abstract

The invention discloses a microbial identification and analysis system and device based on second-generation high-throughput sequencing, and the system comprises a task management module which is usedfor building a task list, managing an identification and analysis task in the task list, uploading to-be-identified and analyzed data to a server, and carrying out the identification and analysis ofthe to-be-identified and analyzed data through the server; a report management module used for generating an identification and analysis report according to the identification and analysis result andcompleting the auditing and approving process of the identification and analysis report according to an auditing and approving instruction of a user; and a report statistics module used for performingreport statistics on the identification analysis report or the identification analysis sample according to the four dimensions of the strain, the microorganism type, the strain source and the identification analysis report statistics. According to the invention, strain or strain identification can be carried out at a molecular level, and the identification result is more accurate and the throughput is high; besides conventional bacteria and fungi, mould and actinomycetes can be identified; meanwhile, mixed bacteria or bacteria difficult to culture can be identified; and the whole genome sequencing data can be subjected to traceability analysis.

Description

technical field [0001] The invention relates to the technical field of genome sequencing, in particular to a microbial identification and analysis system and device based on second-generation high-throughput sequencing. Background technique [0002] In the fields of biomedicine, food safety, and disease diagnosis, the accurate and rapid identification of microorganisms plays a key role. At present, most microbial identification methods are based on the principles of traditional morphology and physiological and biochemical reactions. These detection methods are easily affected by the culture conditions and growth status of the bacteria, and the repeatability of the identification results is poor. Identification. [0003] The second-generation high-throughput sequencing technology is based on the first-generation sequencing technology. It can sequence hundreds of thousands to millions of DNA molecules at a time. It has revolutionized the first-generation sequencing. The next-...

AI Technical Summary

Problems solved by technology

[0008] For the existing high-throughput detection methods of microorganisms, since the detection principle is based on the physiological and biochemical characteristics of microorganisms, it is impossible to identify molds and actinomycetes, and microorganisms that require strict culture conditions cannot be identified; and the judgment of the identification results is subjective. Reproducibility, poor repeatability of test results, unable to carry out large-scale microbial identification, the present invention provides the following technical solutions:

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