Recombinant escherichia coli for producing N-acetyl-5-hydroxytryptamine, and construction method and application of recombinant escherichia coli

A technology for recombining Escherichia coli and Escherichia coli, which is applied in the field of genetic engineering, can solve the problems of expression, low expression of inclusion bodies, and low protein specific activity

Active Publication Date: 2020-06-19
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the synthesis pathway of tryptophan derivatives is very clear, the proteins involved in it have low specific activity, and most of them are difficult to express in the existing mature expr

Method used

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  • Recombinant escherichia coli for producing N-acetyl-5-hydroxytryptamine, and construction method and application of recombinant escherichia coli
  • Recombinant escherichia coli for producing N-acetyl-5-hydroxytryptamine, and construction method and application of recombinant escherichia coli
  • Recombinant escherichia coli for producing N-acetyl-5-hydroxytryptamine, and construction method and application of recombinant escherichia coli

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Embodiment 1

[0125] Embodiment 1, the construction of the recombinant Escherichia coli strain with high yield of N-acetyl-5-hydroxytryptamine

[0126] 1. PCR amplification of the coding sequence of proteins related to the synthesis of tryptophan derivatives

[0127] Using the synthetic phenylalanine hydroxylase gene (shown in positions 319-1209 of SEQ ID NO.1) derived from Xanthomonas campestris as a template, PCR amplification was performed with primers F1 and R1 to obtain a phenylalanine hydroxylase gene containing benzene PCR amplification product X of alanine hydroxylase gene (represented by positions 299-1232 of SEQ ID NO.1).

[0128] Using the synthetic 4a-hydroxytetrahydrobiopterin dehydratase gene phhB (shown in 1234-1590 of SEQ ID NO.1) derived from Pseudomonas aeruginosa as a template, PCR amplification was performed with primers F3 and R3 to obtain PCR amplification product P of 4a-hydroxytetrahydrobiopterin dehydratase gene phhB (represented by positions 1210-1614 of SEQ ID NO...

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Abstract

The invention discloses recombinant escherichia coli for producing N-acetyl-5-hydroxytryptamine, and a construction method and application of the recombinant escherichia coli. The invention firstly discloses the recombinant escherichia coli, and compared with a receptor escherichia coli, the recombinant escherichia coli has the advantages that the expression level of a gene of a tryptophan derivative synthesis related protein is increased and/or the content of the tryptophan derivative synthesis related protein is increased and/or the activity of the tryptophan derivative synthesis related protein is increased, wherein the tryptophan derivative synthesis related protein is selected from at least one of phenylalanine hydroxylase, dihydromonophosphate reductase, 4[alpha]-hydroxytetrahydrobiopterin dehydratase, aspartate transaminase family proteins and N-acetyl transferase. The invention further discloses the construction method and application of the recombinant escherichia coli. The N-acetyl-5-hydroxytryptamine synthesis efficiency of the recombinant escherichia coli for producing the N-acetyl-5-hydroxytryptamine is obviously higher than synthesis efficiency of other existing strains, and the recombinant escherichia coli has great significance for industrial production and large-scale application of N-acetyl-5-hydroxytryptamine.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a recombinant Escherichia coli producing N-acetyl-5-serotonin and its construction method and application. Background technique [0002] N-acetyl-5-hydroxytryptamine (N-Acetyl-serotonin, C 12 h 14 N 2 o 2 ) is a derivative of tryptophan and is the precursor of melatonin synthesis. Its synthesis pathway widely exists in natural organisms. The classic synthesis pathway in cells is: tryptophan—5-hydroxytryptophan—5-hydroxytryptamine — N-acetyl-5-hydroxytryptamine. N-acetyl-5-hydroxytryptamine plays many important roles in organisms. As a precursor of melatonin, N-acetyl-5-hydroxytryptamine also has good antioxidant capacity, such as effective protection against photocarcinogenesis caused by ultraviolet light and DNA damage, while N-acetyl-5-hydroxytryptamine plays an important role in the recovery of brain injury / stroke / nerve damage, and has the function of a neuroprotective ...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/70C12P17/10C12R1/19
CPCC12N9/0071C12N9/1096C12Y402/01096C12Y206/01001C12N9/88C12N9/1025C12N9/0006C12N15/70C12P17/10
Inventor 董志扬张岩峰张山何永志张楠
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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