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Stable coupling dye for photometric determination of analytes

A technology of analytes and dyes, which can be used in the determination/inspection of microorganisms, analytical materials, azo dyes, etc., and can solve the problems of inactivity

Inactive Publication Date: 2003-07-02
LIFESCAN INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many of these commercially available enzymes have little or no activity at this low pH

Method used

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  • Stable coupling dye for photometric determination of analytes
  • Stable coupling dye for photometric determination of analytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Unlike MBTH, these derivatives are quite stable even when heated at 100°C for up to 16 hours, whether in the acid-free or acid-hydrated form. In addition, peroxide-catalyzing enzymes, such as horseradish peroxidase, are particularly effective at reversing oxidative coupling reactions under oxidative reaction conditions. Embodiment 1-synthesis of MBTH derivatives

[0025] Synthesis of m-[3-methyl 2-benzothiazolinone hydrazone] N-sulfonylbenzenesulfonic acid monosodium [2] synthetic scheme

[0026] raw material

[0027] 3-Methyl 2-benzothiazolinone hydrazone hydrochloride (MBTH·HCl), NaI, tetrabutylammonium hydroxide, dichloromethane, and N-methyl-2-pyrrolidone were purchased from Aldrich Company of Milwaukee, Wisconsin , used without purification. Triethylamine is manufactured by Baker Chemicals and distributed by Baxter Company of Phillipsburg (New Jersey). 1,3-Disulfonylchlorobenzene was purchased from Fluka Chemicals of Ronkonkoma, New York or Lancaster Chemical...

Embodiment 2

[0033] Embodiment 2 prepares test device

[0034] Aqueous impregnating solution

(Use NaOH to

pH adjusted to 4.25)

Organic Soaking Solution

water

20ml

water

3ml

citric acid

420mg

industrial alcohol

7ml

Ethylenediaminetetraacetic acid

(EDTA)

16.7mg

m-[3-methyl-2-benzo

Thiazolone Hydrazone] N-Sulphon

Monosodium Acylbenzenesulfonate[2]

10-60mg

Gantrez S95

(purchased from GAF, NewYork,

New York)

90mg

ANS

10-100mg

Crotein SPA (obtained from

Croda Co.,

New York, New York)

250mg

Glucose oxidase

20,500

unit

horseradish peroxidase

16,200

unit

[0035] A glucose-containing blood sample is applied to the surface of the reagent-soaked pad. The sample was immediately absorbed into the substrate and then appeared blue. The intensity of the color increases with ...

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Abstract

A dye couple compound is provided for use in a test device containing a reagent system for detecting the presence or quantity of an analyte in a sample. The reagent system comprises one or more enzymes which, in the presence of the analyte, produce an oxidizing agent in quantities indicative of the quantity of analyte in the sample. The compound of choice is meta[3-methyl 2-benzothiazolinone hydrozone]N-sulfonyl benzenesulfonate monosodium.

Description

technical field [0001] The present invention is an invention relating to assay devices and methods for the colorimetric determination of chemical and biochemical constituents (analytes) in aqueous fluids such as whole blood, and more particularly to coupling dyes for use in such devices and methods . Background technique [0002] The quantitative analysis of the chemical and biochemical constituents of colored aqueous fluids, especially colored biological fluids such as whole blood and urine, and derivatives of biological fluids such as serum and plasma, is becoming increasingly important. It has important uses in medical diagnosis and treatment, and in the quantification of exposure to therapeutic agents, anesthetics, dangerous chemicals, etc. In some cases, the amount of substance being measured is so small—in the mg / dl range or less—or so difficult to measure accurately that the equipment used is complex and only skilled experimenters before use. In this case, results ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N31/22C07D277/82C09B55/00C12Q1/26C12Q1/28C12Q1/54G01N21/78G01N33/533G01N33/535
CPCC12Q1/26Y10T436/143333
Inventor Y·S·玉
Owner LIFESCAN INC