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Analytical element and method for determination of specific binding ligand using vanadium bromoperoxidase as signal-generating enzyme

A vanadium bromoperoxide, specific technology, is applied in the direction of material analysis by observing the effect on chemical indicators, biochemical equipment and methods, and analysis by chemical reaction of materials, etc., to achieve stable enzyme detection, enhanced The effect of detection

Inactive Publication Date: 2003-07-02
ORTHO-CLINICAL DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Although analytical elements and methods disclosed in the prior art show improved test sensitivity and robustness, further improvements are still needed in this field

Method used

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  • Analytical element and method for determination of specific binding ligand using vanadium bromoperoxidase as signal-generating enzyme
  • Analytical element and method for determination of specific binding ligand using vanadium bromoperoxidase as signal-generating enzyme
  • Analytical element and method for determination of specific binding ligand using vanadium bromoperoxidase as signal-generating enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1 Preparation of aqueous chemiluminescence composition and vanadium bromoperoxidase solution

[0097] The aqueous composition providing the chemiluminescent signal was formulated as follows:

[0098] A buffer solution (pH 8.0) containing 100 μM diethylenetriaminepentaacetic acid (DTPA), 0.75M NaBr, and 1% cetyltrimethylammonium bromide (CTAB) in 0.05M Tris-HCl buffer was prepared.

[0099] Signal producing compositions were prepared from two mixtures. The first part contained 1 mM luminol sodium, while the second part contained 1 mM H 2 o2 , both prepared from the above buffer. The two copies were combined just before use.

[0100] A stock solution of vanadium bromoperoxidase (obtained from Ascophyllum nodosum) stored at 4°C was serially diluted in 0.05M Tris-HCl, pH 8 and 0.01% bovine serum albumin (BSA) to provide known concentrations ranging from 10 to 50000×10 -18 moles of vanadium bromoperoxidase.

[0101] Coated elements...

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Abstract

An analytical element can be used to sensitively and rapidly detect a wide variety of specific binding ligands in either a competitive or sandwich assay format. The assays are carried out using a vanadium bromoperoxidase-labeled immunoreactant and a chemiluminescent signal-providing wash composition which comprises a 2,3-dihydro-1,4-phthalazinedione derivative; a halogen, pseudohalogen, halogen-providing source or pseudohalogen-providing source; and a peroxide or a peroxide-generating reagent composition.

Description

technical field [0001] The invention relates to an analytical element and an analytical method for the detection of a specific binding partner in a liquid sample. More specifically, the present invention relates to the use of vanadium bromoperoxidase as the signal-generating enzyme in the assay element. Background technique [0002] In medical practice and research, as well as in analytical and diagnostic procedures, there is a constant need for the rapid and accurate determination of chemical and biological substances present in various fluids, such as biological fluids. For example, rapid and accurate determination of the presence of proteins, hormones, drugs, viruses, microorganisms, narcotics, and steroids, etc., is necessary for effective research, diagnosis, and treatment. [0003] In recent decades, many different analytical methods have been developed to detect the above substances. These methods are already reliable and, in some cases, suitable for automation and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/535C12Q1/28G01N21/77G01N33/543
CPCG01N33/54386Y10S436/805Y10S436/81G01N33/543C12Q1/28
Inventor A·E·弗里德曼T·R·基瑟尔S·F·格劳克斯M·M·科思恩斯基
Owner ORTHO-CLINICAL DIAGNOSTICS
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