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Method for inducing and breeding high-yield cotton by using Asian cotton pollen

An Asian cotton and cotton technology, applied in the field of cotton breeding, can solve the problems of low efficiency of cotton hybrid breeding, achieve the effects of improving breeding efficiency, speeding up the breeding process, and simple operation

Inactive Publication Date: 2020-06-26
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of low efficiency of cotton hybrid breeding, and provides a method for inducing diploid Asian cotton powder and chemical reagents to induce cotton double haploid, which can improve the frequency and efficiency of cotton haploid induction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for inducing and breeding high-quality and high-yielding cotton utilizing Asian cotton flour, comprising the steps of:

[0026] (1) The combination of upland cotton Fengfeng product line 4133Bt and high-quality line E0904, the flower buds of F1 were emasculated one day before flowering, and marked.

[0027] (2) Take the flowering pollen of Asia No. 1 in the morning of the same day, and smear it on the emasculated F1 stigma. On the day of flowering, dip a writing brush in dimethyl sulfoxide (DMSO) 45ml L -1 , colchicine 4.5g L -1 , Inositol 100mg L -1 , indole acetic acid 25mg L -1 , 2,4-D 25mg L -1 , 25mg / L NAA, 40mg / L gibberellin inducer, repeatedly smeared on the ovary of the stamen-removed flower, after smearing, the style is covered with a wax tube to prevent exogenous pollen, and it is applied again the next day after flowering.

[0028] (4) On the 10th day after flowering, pick off the enlarged ovary, clean the epidermis, soak it with 75% alcohol sol...

Embodiment 2

[0030] A method for inducing and breeding high-quality and high-yielding cotton utilizing Asian cotton flour, comprising the steps of:

[0031] (1) Cotton abundant product line 4133Bt and Sea Island cotton high-yield high-quality line Hai 7124 are configured and combined, and the flower buds of F1 are emasculated one day before flowering, and are listed and marked

[0032] (2) Take the flowering pollen of Asia No. 1 in the morning of the same day, and smear it on the emasculated F1 stigma. On the day of flowering, dip a writing brush in dimethyl sulfoxide (DMSO) 45ml L -1 , colchicine 4.5g L -1 , Inositol 100mg L -1 , indole acetic acid 25mg L -1 , 2,4-D 25mg L -1 , 25mg / L NAA, 40mg / L gibberellin inducer, repeatedly smeared on the ovary of the stamen-removed flower, after smearing, the style is covered with a wax tube to prevent exogenous pollen, and it is applied again the next day after flowering.

[0033] (3) On the 10th day after flowering, pick off the enlarged ovary...

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Abstract

The invention belongs to the field of cotton seedling and particularly relates to a method for inducing and breeding high-yield cotton by using Asian cotton pollen. The method comprises the steps of emasculating cotton pollen; pollinating Asian cotton pollen; removing ovaries of stamens by inducer coating; selecting the ovaries which expand at 10 days after anthesis, and taking out ovules to culture on an induction medium until buds grow; and after seedling roots are appropriate, transplanting seedlings into a plantlet hardening medium. The method for inducing and breeding the high-yield cotton by using the Asian cotton pollen provided by the invention is simple in operation; the Asian cotton pollen is utilized to induce expansion of the ovaries, macrospores are doubled under the inductionof dimethyl sulfoxide, colchicine and the like, and the ovules which can normally expand are successfully induced euploids; the induction efficiency of double haploid in the cotton is improved; heterosis between tetraploid cotton can be quickly fixed; and the breeding efficiency of the cotton is improved.

Description

technical field [0001] The invention belongs to the field of cotton breeding, and in particular relates to a method for inducing high-quality and high-yield cotton by using Asian cotton powder. Background technique [0002] Cotton is the most important fiber crop in my country, and its production status has an important impact on the development of the national economy. With the continuous acceleration of the urbanization process and the adjustment of the agricultural structure in recent years, the cotton planting area in my country has been shrinking, and the cotton planting area will gradually shift to coastal saline land, northwest dry land, and saline land, and the benefits of cotton planting are also declining year by year. On the other hand, there is a lack of innovation in my country's cotton varieties, single variety types, poor disease resistance and stress resistance, and the improvement of fiber quality and yield has been stagnant, unable to meet the needs of prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08A01H4/00
CPCA01H1/02A01H1/08A01H4/008
Inventor 贾银华杜雄明潘兆娥何守朴耿晓丽王立如庞保印李洪戈陈保军
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI