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A kind of extraction method of betulinic acid

An extraction method and technology of betulinic acid, applied in the direction of steroids, organic chemistry, etc., can solve the problems of low yield, large solvent consumption, and difficult separation of impurities.

Active Publication Date: 2021-02-12
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method of extracting and separating betulinic acid from natural plants has the following disadvantages: a large amount of solvent is consumed, the yield is low, impurities are not easy to separate, and the purity is low.

Method used

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  • A kind of extraction method of betulinic acid
  • A kind of extraction method of betulinic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A method for extracting betulinic acid, comprising the following steps:

[0069] Add water to 500g of sycamore bark for ultrasonic treatment, the number of times of adding the water every 24h is 3 times, the amount of water added each time is 12mL / g of sycamore bark, the temperature of water is 22 ℃, and the ultrasonic wave The power is 2500W, the frequency is 35kHz, the number of times of the ultrasonic treatment per 24h is 3 times, and the time of each treatment is 7min; the bark after the ultrasonic treatment is extracted with a composite solvent, and in each gram of the bark The volume of the composite solvent added is 1-2.5mL, the composite solvent is a mixed solution of methanol, ethanol, butanol and acetone, and the mass ratio of methanol, ethanol, butanol and acetone in the composite solvent is 4:3:2 : 1, the temperature of the leaching is 23 ℃, the number of times of the leaching is 4 times, and the time of each leaching is 25h, and the volume percentage of wat...

Embodiment 2

[0071] A method for extracting betulinic acid, comprising the following steps:

[0072] Add water to 420g of sycamore bark for ultrasonic treatment, the number of times of adding the water every 24h is 1 time, and the amount of water added each time is 10mL / g of sycamore bark, the temperature of the water is 10°C, and the ultrasonic The power is 1100W, the frequency is 40kHz, the number of times that the ultrasonic wave is processed every 24h is 1 time, and the time of each processing is 8min; Adopt composite solvent to extract the bark after ultrasonic treatment, described composite solvent is methanol, The mixed solution of ethanol, butanol and acetone, the volume that adds described complex solvent in every gram described bark is 2.5mL, and the mass ratio of methanol, ethanol, butanol and acetone is 4:4:1:1 in the described complex solvent , the temperature of the leaching is 21°C, the number of times of the leaching is 3 times, the time of each leaching is 10h, the volume ...

Embodiment 3

[0074] A method for extracting betulinic acid, comprising the following steps:

[0075] Water was added to 470g of sycamore bark for ultrasonic treatment, and the number of times of adding the water every 24h was 2 times, and the amount of water added each time was 15mL / g of sycamore bark, and the temperature of the water was 30°C. The power is 3500W, the frequency is 20kHz, the number of times that the ultrasonic wave is processed every 24h is 2 times, and the time of each processing is 4min; Adopt composite solvent to extract the bark after ultrasonic treatment, described composite solvent is methanol, The mixed solution of ethanol, butanol and acetone, the volume that adds described composite solvent in the described bark of every gram is 1mL, and the mass ratio of methyl alcohol, ethanol, butanol and acetone is 3:4:1:2 in the described composite solvent, The temperature of the leaching is 26°C, the number of times of the leaching is 1 time, the time of each leaching is 30h...

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Abstract

The invention relates to the technical field of extraction of traditional Chinese medicines, in particular to a method for extracting betulinic acid. The extraction method of betulinic acid in the present invention comprises the following steps: ultrasonically treating the mixture of sycamore bark and water, using a composite solvent to extract the ultrasonically treated bark, and filtering the obtained extracts in sequence , concentration and drying; in the mixture of sycamore bark and water, the temperature of the water is 10-30°C; the composite solvent includes an organic solvent and water; the organic solvent includes methanol, ethanol, butyl At least one of alcohol and acetone; the volume percentage of water in the composite solvent is 10% to 20%. In the invention, the bark of the sycamore family is mixed with water at a specific temperature for ultrasonic treatment, and then extracted with a specific composite solvent, so that the obtained betulinic acid has higher yield and purity.

Description

technical field [0001] The invention relates to the technical field of extraction of traditional Chinese medicines, in particular to a method for extracting betulinic acid. Background technique [0002] Betulinic acid can be extracted from rose apple leaves, white birch bark, jujube seed, and can also be obtained by chemical synthesis using betulin as raw material. Studies have found that betulinic acid can selectively kill human melanoma cells without killing healthy cells; betulinic acid can inhibit HIV-1 infection. Malignant tumor cells such as germ layer tumor and leukemia also have inhibitory effect. [0003] At present, the method of extracting and separating betulinic acid from natural plants has the following disadvantages: a large amount of solvent is consumed, the yield is low, impurities are not easy to separate, and the purity is low. [0004] In view of this, the present invention is proposed. Contents of the invention [0005] According to one aspect of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 任浩沈人杰刘洁翟华敏
Owner NANJING FORESTRY UNIV