Kiwifruit in-vitro rapid seedling culture method
A kiwifruit, fast technology, applied in the field of kiwifruit seedlings, can solve the problems of underdeveloped root system, seedlings susceptible to diseases, etc., achieve the effect of strong adaptability of seedlings, and solve the effect of easily infectious bacteria
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[0022] A kind of kiwifruit in vitro rapid seedling cultivation method comprises the following steps:
[0023] S1: Cultivate virus-free scion seedlings
[0024] The female parent of the detoxified scion seedling adopts the stem tip detoxification method to induce, multiply and take root to obtain the detoxified scion seedling. The female parent of the detoxified scion seedling in this embodiment is Actinidia chinensis Planch. One or two kinds of kiwifruit (Actinidia chinensis Planch.), the female parents of these two virus-free scion seedlings are Jinggangshan wild kiwifruit, and these two kinds of kiwifruit are naturally distributed in the shrubs at an altitude of 200-2100 meters in Jinggangshan. Strong, the fruit is better; the medium used in the induction stage is based on MS medium, which also contains 3mg / L 6-BA, 0.2mg / LNAA and 30g / L sucrose; the medium used in the proliferation stage is based on MS medium It is the basal medium, which also contains 0.5mg / L 6-BA, 0.1mg / LN...
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