Method and system for treating polyhydroxyalkanoate fermentation liquid by membrane filtration and application of obtained fermentation waste liquid
A technology of polyhydroxyalkanoate and treatment method, which is applied in the field of application of fermentation serum in PHA fermentation, can solve the problems of high substrate and sterilization cost, high production cost, high production cost, etc., and achieve effective separation, The effect of reducing the cost of auxiliary materials and reducing production costs
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[0044] According to the present invention, the fermentation liquid of the polyhydroxyalkanoate can be the fermentation liquid of microorganisms that can be used to prepare polyhydroxyalkanoate conventionally in the art, preferably, the microorganism is a halophilic bacteria, for example, it can be a salt One of the genus Monas, according to a preferred embodiment of the present invention, the PHA fermentation strain is Halomonas sp.; more preferably, the PHA fermentation strain is Halomonas sp. Bacteria (Halomonas sp.) TD01, its preservation number is CGMCCNO.4353 (CN201010578858.8).
[0045] According to the present invention, the conditions of the PHA fermentation can be conventional PHA fermentation conditions in the art, for example, the inoculum of 5-20 volume % is inserted into the fermentation medium with fresh seed liquid, and the fermentation system is not sterilized and directly fermented . The temperature is controlled at 33-37°C, the initial dissolved oxygen is co...
Embodiment 1
[0087] This embodiment is used to illustrate the processing method of polyhydroxyalkanoate fermented liquid provided by the present invention
[0088] (1) The polyhydroxyalkanoate fermentation liquid prepared by the preparation example is centrifuged through a disc centrifuge, and the fermentation liquid is divided into a bottom flow rich in fermentation cells and a top flow of the fermentation raffinate (solid content 7% by weight), The top flow of the fermentation raffinate enters the fermentation raffinate temporary storage tank for further processing, and the bottom flow of the fermentation cell enters the extraction tank for PHA extraction.
[0089] (2) pump the liquid phase in the temporary storage tank of the fermentation raffinate into the plate-and-frame filter to filter, further separate the residual thallus and solid suspended matter, and send the filtered solid phase into the extraction tank and step (1) ) together with the next step of PHA extraction, and the liqu...
Embodiment 2
[0092] This embodiment is used to illustrate the processing method of polyhydroxyalkanoate fermented liquid provided by the present invention
[0093] (1) The polyhydroxyalkanoate fermentation liquid prepared by the preparation example is centrifuged through a disc centrifuge, and the fermentation liquid is divided into an underflow rich in fermentation cells and a top flow of the fermentation raffinate (solid content 10% by weight), The top flow of the fermentation raffinate enters the fermentation raffinate temporary storage tank for further processing, and the bottom flow of the fermentation cell enters the extraction tank for PHA extraction.
[0094] (2) pump the liquid phase in the temporary storage tank of the fermentation raffinate into the plate-and-frame filter to filter, further separate the residual thallus and solid suspended matter, and send the filtered solid phase into the extraction tank and step (1) ) together with the next step of PHA extraction, and the liqu...
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