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Method and system for treating polyhydroxyalkanoate fermentation liquid by membrane filtration and application of obtained fermentation waste liquid

A technology of polyhydroxyalkanoate and treatment method, which is applied in the field of application of fermentation serum in PHA fermentation, can solve the problems of high substrate and sterilization cost, high production cost, high production cost, etc., and achieve effective separation, The effect of reducing the cost of auxiliary materials and reducing production costs

Active Publication Date: 2020-12-11
JILIN COFCO BIOCHEM +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of PHA can effectively avoid the harm caused by petrochemical plastics to the environment, at present, the production cost of this environmentally friendly bioplastic is high, and the commercial development of PHA has been restricted by its high production cost. In order to reduce costs, research Researchers have been working on solving the problems of high cost of substrate and sterilization, low yield, etc.
[0003] Selecting high-quality, high-yielding, non-infectious chassis bacteria is an effective way to improve quality and reduce costs. Halophilic bacteria are high-quality and high-yielding strains that have been bred in recent years. Although they have reduced the production cost of PHA to a certain extent, but Its fermentation process requires a high concentration of salt environment
Due to the use of a large amount of inorganic salts, on the one hand, it will also lead to high production costs, on the other hand, the high-salt wastewater produced is also facing the problem that it needs to be treated at a high cost

Method used

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  • Method and system for treating polyhydroxyalkanoate fermentation liquid by membrane filtration and application of obtained fermentation waste liquid
  • Method and system for treating polyhydroxyalkanoate fermentation liquid by membrane filtration and application of obtained fermentation waste liquid

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Embodiment approach

[0044] According to the present invention, the fermentation liquid of the polyhydroxyalkanoate can be the fermentation liquid of microorganisms that can be used to prepare polyhydroxyalkanoate conventionally in the art, preferably, the microorganism is a halophilic bacteria, for example, it can be a salt One of the genus Monas, according to a preferred embodiment of the present invention, the PHA fermentation strain is Halomonas sp.; more preferably, the PHA fermentation strain is Halomonas sp. Bacteria (Halomonas sp.) TD01, its preservation number is CGMCCNO.4353 (CN201010578858.8).

[0045] According to the present invention, the conditions of the PHA fermentation can be conventional PHA fermentation conditions in the art, for example, the inoculum of 5-20 volume % is inserted into the fermentation medium with fresh seed liquid, and the fermentation system is not sterilized and directly fermented . The temperature is controlled at 33-37°C, the initial dissolved oxygen is co...

Embodiment 1

[0087] This embodiment is used to illustrate the processing method of polyhydroxyalkanoate fermented liquid provided by the present invention

[0088] (1) The polyhydroxyalkanoate fermentation liquid prepared by the preparation example is centrifuged through a disc centrifuge, and the fermentation liquid is divided into a bottom flow rich in fermentation cells and a top flow of the fermentation raffinate (solid content 7% by weight), The top flow of the fermentation raffinate enters the fermentation raffinate temporary storage tank for further processing, and the bottom flow of the fermentation cell enters the extraction tank for PHA extraction.

[0089] (2) pump the liquid phase in the temporary storage tank of the fermentation raffinate into the plate-and-frame filter to filter, further separate the residual thallus and solid suspended matter, and send the filtered solid phase into the extraction tank and step (1) ) together with the next step of PHA extraction, and the liqu...

Embodiment 2

[0092] This embodiment is used to illustrate the processing method of polyhydroxyalkanoate fermented liquid provided by the present invention

[0093] (1) The polyhydroxyalkanoate fermentation liquid prepared by the preparation example is centrifuged through a disc centrifuge, and the fermentation liquid is divided into an underflow rich in fermentation cells and a top flow of the fermentation raffinate (solid content 10% by weight), The top flow of the fermentation raffinate enters the fermentation raffinate temporary storage tank for further processing, and the bottom flow of the fermentation cell enters the extraction tank for PHA extraction.

[0094] (2) pump the liquid phase in the temporary storage tank of the fermentation raffinate into the plate-and-frame filter to filter, further separate the residual thallus and solid suspended matter, and send the filtered solid phase into the extraction tank and step (1) ) together with the next step of PHA extraction, and the liqu...

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Abstract

The invention relates to the field of preparation of bio-based material polyhydroxyalkanoate, and in particular, relates to a method and system for treating polyhydroxyalkanoate fermentation liquor through membrane filtration. The method comprises the steps: carrying out solid-liquid separation on fermentation liquor of polyhydroxyalkanoate to obtain fermentation clear liquor; and then carrying out membrane filtration on the fermentation clear liquid to remove impurities in the fermentation clear liquid so as to obtain treated fermentation clear liquid. The system comprises a first solid-liquid separation unit and a membrane filtration unit, the first solid-liquid separation unit is used for carrying out solid-liquid separation on a fermentation broth of polyhydroxyalkanoate to obtain thefermentation clear liquid; the membrane filtration unit is used for carrying out membrane filtration on the fermentation clear liquid so as to remove the impurities in the fermentation clear liquid, so that the treated fermentation clear liquid is obtained and is reused in the next fermentation. According to the method and the system, the production cost of PHA can be effectively reduced, and thefermentation efficiency and the PHA yield of PHA fermentation strains can be effectively guaranteed.

Description

technical field [0001] The invention relates to the field of preparation of biological substrate polyhydroxyalkanoate, in particular to a method and a treatment system for treating polyhydroxyalkanoate fermentation liquid by membrane filtration, and the obtained treated fermentation liquid is fermented in PHA in the application. Background technique [0002] Polyhydroxyalkanoate (PHA) is a general term for a class of polymer polyesters that are completely synthesized by microorganisms. PHA is biodegradable and biocompatible, so it is considered as an environmentally friendly material, which helps to solve the increasingly serious environmental pollution problem. Although the use of PHA can effectively avoid the harm caused by petrochemical plastics to the environment, at present, the production cost of this environmentally friendly bioplastic is high, and the commercial development of PHA has been restricted by its high production cost. In order to reduce costs, research R...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/02C12P7/62C12R1/01C02F103/38
CPCC02F1/001C02F1/38C02F1/444C02F9/00C02F2103/38C02F2301/08C12P7/625
Inventor 佟毅李义田芳许克家郭元亨刘安妮陈博安泰李大勇刘海军武丽达
Owner JILIN COFCO BIOCHEM