A movable chip holding device for nucleic acid amplification instrument

Abstract

The invention relates to a movable chip holding device for a nucleic acid amplification instrument. The nucleic acid amplification instrument includes a movable chip holding device. When a nucleic acid amplification reaction occurs, the chip in the movable chip holding device The center is facing the camera and the light source; the movable chip clamping device includes a chip clamp and a slide module assembly; the chip clamp is arranged on the slide module assembly, and a temperature control module is arranged on the chip clamp , the temperature control module includes a temperature control adapter board, the chip clamp includes a chip cover, a chip pressing plate, a chip frame and a chip positioning piece, and the slide module assembly includes a slider, a ball screw, a coupling Shaft and motor assembly, the present invention aligns the chip with the positioning groove, fastens the chip gland, and the chip pressing plate will be close to the top of the chip during the fastening process, causing the chip pressing plate to be pressed by the spring force of the chip, resulting in a soft connection The effect is to make the bottom of the chip closely connected with the thermal pad.

Description

Technical field [0001] The invention belongs to the technical field of nucleic acid amplification, and particularly relates to a movable chip clamping device for a nucleic acid amplification instrument. Background technique [0002] PCR chip technology (Microfluidics) integrates basic operation units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis process into a micron-scale chip to automatically complete the entire analysis process. The chip and the chip fixture are used together to facilitate sample introduction and experimental observation. Therefore, the reliable connection between the chip and the chip fixture is very important. Similarly, the chip fixture should ensure the tightness of the chip so that the reaction is not affected by the outside. [0003] Patent application document CN105413779A discloses a microfluidic chip fixture for preparing multiple emulsions and a system for preparing multiple emulsions....

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned existing technical problems, in order to solve the complex chip picking and placing process, the problem of chip heating stability, and simplify the alignment process of the chip and the lens light source; the invention provides a movable chip clamping device for a nucleic acid amplification instrument , the nucleic acid amplification instrument includes a movable chip holding device, when nucleic acid amplification reaction occurs, the center of the chip in the movable chip holding device faces the camera and the light source; the movable chip holding device includes a chip holding device . Slider module assembly; the chip fixture is arranged on the slide module assembly, the chip fixture is provided with a temperature control module, the temperature control module includes a temperature control module outer frame, and the chip fixture includes A chip gland, a chip pressing plate, a chip frame, and a chip positioning piece, the chip gland is slidingly connected to the outer frame of the temperature control module, the outer frame of the temperature control module is provided with a positioning groove, and the chip positioning piece Installed in the positioning groove, the chip frame is placed on the chip positioning sheet, the chip frame is provided with a mounting groove, the chip is installed in the mounting groove, and the chip pressing plate is installed on the chip gland through 4 screws Above, each of the screws is sleeved with a compression spring; when the chip gland presses down on the chip in the chip frame, the chip pressing plate moves upwards, so that the chip and the temperature control module are in better contact;

Images