Application of onjisaponin F in preparation of drug for preventing and treating huntington disease
A technology of polygala saponin and Huntington's disease, which is applied in the field of medicine and can solve problems such as no treatment methods
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[0038] (1) The degradation effect of polygala saponin F on EGFP-HDQ74 transfected by mouse neuroblastoma (Neuro2a) cells. The specific process is as follows.
[0039] 1. Cell culture: subculture at a ratio of 1:5 each time
[0040] (1) Aspirate and discard the culture medium, wash once with 3-10 ml 0.01M PBS;
[0041] (2) Add 3-5 ml 0.25% trypsin-EDTA to digest for 2-3 minutes;
[0042] (3) Add 3-5 ml DMEM medium containing 10% FBS to stop the digestion, and gently pipette to separate the cells;
[0043] (4) Aspirate the cell suspension in the dish into a 15ml centrifuge tube;
[0044] (5) Centrifuge (1200rpm 2min), and discard the supernatant;
[0045] (6) Add 1ml of DMEM medium containing 10% FBS to resuspend, suck out 200μl to a 10cm petri dish, plant two dishes, suck out 67ul to a 6cm petri dish, and put it in a 37°C incubator with 5% CO2 .
[0046] 2. Cell transfection: The day before transfection, plate two 6-well plates and one 24-well plate
[0047] (1) Take a 1...
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