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Application of platycodon grandiflorum polysaccharide in antagonizing fumonisin B1-induced apoptosis through autophagy

A technology of platycodon polysaccharides and alveolar macrophages, which is applied in the field of medicinal uses of platycodon polysaccharides, and can solve the problems of unpredictable effects of cell damage, unpredictability, and cell apoptosis

Active Publication Date: 2020-07-03
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, the molecular weight, chemical composition, glycosidic bond, conformation, branching degree, etc. of polysaccharides will affect the activity of polysaccharides. Therefore, the activity of polysaccharides is different and unpredictable; on the other hand, different types Different inducers have different mechanisms of cell damage, therefore, even the same substance has unpredictable effects on cell damage caused by different inducers
So far, there is no report about platycodon polysaccharides antagonizing fumonisin B1-induced apoptosis through autophagy

Method used

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  • Application of platycodon grandiflorum polysaccharide in antagonizing fumonisin B1-induced apoptosis through autophagy
  • Application of platycodon grandiflorum polysaccharide in antagonizing fumonisin B1-induced apoptosis through autophagy
  • Application of platycodon grandiflorum polysaccharide in antagonizing fumonisin B1-induced apoptosis through autophagy

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Embodiment 1: Preparation of Platycodon grandiflorum polysaccharide PGSt

[0053] Cut platycodon root into slices, add 10 times the weight of water to soak overnight, then extract at 90°C for 3 hours, filter, concentrate the filtrate to a relative density of 1.05-1.15 (measured at 50°C), and centrifuge to remove impurities to obtain the extract liquid;

[0054] Add ethanol with a volume concentration of 95% to the extract, stir while adding, so that the final concentration of ethanol is 70%, let stand, centrifuge, separate the precipitate, and dry the precipitate at 60°C to prepare PGPS 70 Add ethanol with a volume concentration of 95% to the supernatant after separation and precipitation, and stir while adding, so that the final concentration of ethanol is 50%, let stand, centrifuge, separate and precipitate, and dry the precipitate at 60°C to prepare PGPS 50 ; the PGPS 70 and PGPS 50 Mix evenly to prepare platycodon polysaccharide PGSt.

Embodiment 2

[0055] Example 2: Platycodon grandiflorum antagonizes fumonisin B1-induced apoptosis of 3D4 / 21 cells through autophagy

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Abstract

The invention discloses an application of platycodon grandiflorum polysaccharide in antagonizing fumonisin B1-induced apoptosis through autophagy. It has been discovered that the toxic effect of FB1 on alveolar macrophages is mainly reflected in induction of apoptosis. The platycodon grandiflorum polysaccharide induces the autophagy through two signal pathways, namely PI3K / Akt / mTOR and MEK / ERK, soas to antagonize FB1-induced alveolar macrophages apoptosis through the autophagy and achieve the protective effect on the alveolar macrophages. According to the application, a regulatory mechanism for interference of the platycodon grandiflorum polysaccharide between the alveolar macrophages apoptosis and the autophagy is illustrated, and a theoretical basis is provided for development of drugsfor treating corresponding diseases.

Description

technical field [0001] The invention belongs to the field of medical applications of platycodon polysaccharides, in particular to the use of platycodon polysaccharides in antagonizing cell apoptosis induced by fumonisin B1 through autophagy. Background technique [0002] Fumonisin B1 (FB1) is a mycotoxin produced by the fungus Fusarium verticillioides, commonly associated with maize. It produces species-specific and organ-specific toxicities, including leukoencephalomalacia in horses, pulmonary edema in pigs, and liver or kidney damage in most animal species. [0003] Alveolar macrophages are an important part of the lung's resistance to pathogenic infection. When in contact with pathogenic factors, alveolar macrophages stretch out pseudopodia and surround them until they form closed bodies, and then the bodies detach from the cell membrane to form cytoplasm The phagosome finally fuses with the lysosome in the cell, which contains a variety of proteases that can digest path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P11/00A61P31/10A61K36/346
CPCA61K31/715A61K36/346A61P11/00A61P31/10
Inventor 赵晓娜刘建柱程国栋邓干臻李克鑫李克钦刘国涛李雯
Owner SHANDONG AGRICULTURAL UNIVERSITY
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