25-hydroxyvitamin D analysis method based on combination of immune purification and mass spectrometric detection

A hydroxyvitamin and analysis method technology, applied in the field of 25-hydroxyvitamin D analysis based on immunopurification combined with mass spectrometry detection, can solve problems affecting quantification and limiting clinical applications, cross-interference, etc., achieve strong specificity and selectivity, and improve Use efficiency, high flux effect
CN111366646AActive Publication Date: 2020-07-03SUZHOU SYM BIO LIFESCI CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU SYM BIO LIFESCI CO LTD
Publication Date
2020-07-03

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Abstract

The invention discloses a 25-hydroxyvitamin D analysis method based on combination of immune purification and mass spectrum detection. The method mainly comprises steps of preparing a biotin-labeled antibody, preparing a biotin-labeled antibody-coated magnetic bead, enriching and purifying a sample and carrying out mass spectrum detection. The analysis method is advantaged in that high specificityof antibody affinity is utilized, 25-(OH) D can be specifically enriched and purified from a complex biological sample, moreover, respective accurate detection of 25-(OH) D2 and 25-(OH) D3 is realized, the antibody coupled magnetic bead enrichment method is combined with mass spectrum detection, so high flux, sensitivity and accurate quantification are realized, moreover, use efficiency of a liquid phase mass spectrum system is greatly improved.
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Description

technical field

[0001] The invention relates to the technical field of biological detection, in particular to a 25-hydroxyvitamin D analysis method based on immunopurification combined with mass spectrometry detection. Background technique

[0002] 25-hydroxyvitamin D (25-(OH)D), including 25-hydroxyvitamin D2 (25-(OH)D2) and 25-hydroxyvitamin D3 (25-(OH)D3), is the main presence of vitamin D in the body form. The level of serum 25-(OH)D can reflect the storage level of vitamin D in the human body, and it is related to the clinical symptoms of vitamin D deficiency. The detection methods of 25-(OH)D2 and 25-(OH)D3 in biological samples (such as whole blood, serum, urine or saliva) mainly include liquid chromatography-mass spectrometry and immunoassay.

[0003] Before detection by liquid chromatography mass spectrometry, 25-(OH)D2 and 25-(OH)D3 need to be enriched and separated from biological samples. Traditional sample processing methods mainly include precipitation, liqui...

Claims

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