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Novel coronavirus COVID-19 antigen fluorescence detection kit and preparation method thereof

A technology for COVID-19 and coronavirus, applied in the field of novel coronavirus COVID-19 antigen fluorescence detection kit and its preparation, to achieve the effects of improving work efficiency, short technical time consumption and accurate detection

Active Publication Date: 2020-07-03
SHENZHEN ZIJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]There is currently no 2019 novel coronavirus COVID-19 antigen detection product on the market, therefore, there is an urgent need for a rapid, specific and accurate detection of novel coronavirus COVID-19 Viral Antigen Fluorescent Detection Kit

Method used

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  • Novel coronavirus COVID-19 antigen fluorescence detection kit and preparation method thereof
  • Novel coronavirus COVID-19 antigen fluorescence detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Embodiment 1, kit of the present invention and preparation method thereof

[0035] S1. Cleaning and activation of fluorescent microspheres: select fluorescent microspheres with a particle size of 0.1 μm, the wavelengths of the excitation light and emission light of the fluorescent microspheres are 400nm and 505nm, respectively, and place the fluorescent microspheres in a centrifuge tube at a low temperature In an ultracentrifuge, centrifuge at a speed of 12000×g for 10 minutes to form a suspension of fluorescent microspheres. After removing the supernatant, the precipitate is resuspended with 20 mM morpholineethanesulfonic acid solution at pH 5.0, and then treated with ultrasonic waves at 100 W for 50 seconds. Make the concentration of the fluorescent microsphere suspension 10 mg / ml, add 50 μl of carbodiimide and N-hydroxysulfosuccinimide solutions with a concentration of 100 mg / ml in sequence, place in a rotary mixer, and mix at room temperature for 15 minutes. Centrifu...

Embodiment 2

[0044] Embodiment 2, kit of the present invention and preparation method thereof

[0045] S1. Cleaning and activation of fluorescent microspheres: select fluorescent microspheres with a particle size of 0.3 μm, the wavelengths of the excitation light and emission light of the fluorescent microspheres are 550nm and 400nm respectively, place the fluorescent microspheres in a centrifuge tube and place them at a low temperature In an ultracentrifuge, centrifuge at a speed of 12000×g for 20 minutes to form a suspension of fluorescent microspheres. After removing the supernatant, the precipitate is resuspended with 30mM morpholineethanesulfonic acid solution at pH5.5, and then treated with 100W ultrasonic wave for 60s. Make the concentration of the fluorescent microsphere suspension 10 mg / ml, add 50 μl of carbodiimide and N-hydroxysulfosuccinimide solutions with a concentration of 100 mg / ml in sequence, place in a rotary mixer, and mix at room temperature for 15 minutes. Centrifuge ...

Embodiment 3

[0054] Embodiment 3, kit of the present invention and preparation method thereof

[0055] S1. Cleaning and activation of fluorescent microspheres: select fluorescent microspheres with a particle size of 0.5 μm, the wavelengths of the excitation light and emission light of the fluorescent microspheres are 750nm and 750nm respectively, place the fluorescent microspheres in a centrifuge tube and place them at a low temperature In an ultracentrifuge, centrifuge at 12,000×g for 20 minutes to form a suspension of fluorescent microspheres. After removing the supernatant, the precipitate is resuspended with 50mM morpholineethanesulfonic acid solution at pH 6.5, and then treated with ultrasonic waves at 100W for 60s. Make the concentration of the fluorescent microsphere suspension 10 mg / ml, add 50 μl of carbodiimide and N-hydroxysulfosuccinimide solutions with a concentration of 100 mg / ml in sequence, place in a rotary mixer, and mix at room temperature for 15 minutes. Centrifuge at 12...

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Abstract

The invention relates to the technical field of biology, and provides a novel coronavirus COVID-19 antigen fluorescence detection kit and a preparation method thereof. The kit comprises a bottom plate. A sample pad, a combination pad, a coating film and absorbent paper are laid on the bottom plate. The combination pad contains a fluorescent microsphere labeled novel coronavirus COVID-19 Spike protein monoclonal antibody and a fluorescent microsphere labeled goat anti-chicken antibody. The coating film comprises a detection area coating T and a quality control area coating C. The detection areais coated with a novel coronavirus COVID-19 Spike protein monoclonal antibody of which the epitope is different from that of the novel coronavirus COVID-19 Spike protein monoclonal antibody on the combination pad. The quality control area is coated with a chicken IgY antibody. The kit disclosed by the invention can accurately detect whether the novel coronavirus Spike protein exists in a sample or not, so that on-site rapid detection is realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a novel coronavirus COVID-19 antigen fluorescence detection kit and a preparation method thereof. Background technique [0002] The 2019 novel coronavirus COVID-19 belongs to the Orthocoronavirus subfamily (OrthocoronS1virinS1e). Within dozens of days from the discovery that viral pneumonia was caused by the virus, due to population mobility, confirmed cases appeared one after another across the country. The virus has an incubation period of up to 14 days and can be transmitted without symptoms. The National Health Commission has decided to include the novel coronavirus-infected pneumonia in the Class B management of statutory infectious diseases, and adopt prevention and control measures for Class A infectious diseases. [0003] Currently, there is no 2019 novel coronavirus COVID-19 antigen detection product on the market. Therefore, a fast, specific and accurate detection kit for...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/577G01N33/569G01N33/558
CPCG01N33/582G01N33/587G01N33/577G01N33/56983G01N33/558G01N2333/165G01N2469/10Y02A50/30
Inventor 操凤郑志于洪波
Owner SHENZHEN ZIJIAN BIOTECH
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