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A kind of thermophilic actinomycetes and application thereof
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A technology of actinomycetes and bacterial strains, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems that harmful bacteria, insect eggs, and beneficial microorganisms cannot be achieved, and the efficiency is low, so as to promote rapid degradation, improve fertilizer quality, Significant effect on fertilizer quality
Active Publication Date: 2022-06-10
山东中农普宁药业有限公司 +1
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The traditional bio-organic fertilizer production technology is mainly based on anaerobic composting. Most of the fermenting microorganisms used are low-temperature strains. The fermentation temperature is generally controlled at 45°C, which takes a long time (3-6 months) and low efficiency. The effect of removing harmful bacteria and insect eggs cannot be achieved, and beneficial microorganisms often cannot meet the national bio-organic fertilizer standards
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Embodiment 1
[0019] The acquisition of Streptomyces capsularis HWJ001 strain
[0020] 1. Isolation and identification of strains:
[0021] 1. Prepare 100ml of commercial actinomycete solid medium in proportion, and sterilize at 120°C for 30 minutes;
[0022] 2. Weigh 10g of decomposed chicken manure into a triangular flask, add 90ml of physiological saline, stir evenly, shake for 30 minutes in a digital display constant temperature water bath oscillator, and set aside;
[0023] 3. Spread the sterilized culture medium into 6 plates and set aside for later use;
[0024] 4. Dip the samples from step 2 with an inoculating loop and streak them in 6 plates;
[0025] 5. Invert the streaked plate in a 36°C incubator for 72 hours.
[0026] 6. Pick the actinomycetes colonies on the plate for further separation and purification.
[0027] 2. Culture preservation:
[0028] 1. Prepare 100ml of commercial actinomycete culture medium in proportion, and sterilize at 120°C for 30 minutes;
[0029] 2. ...
Embodiment 2
[0065] Application of Streptomyces capsularis HWJ001 strain in the production of organic fertilizer by high temperature fermentation of chicken manure.
[0066] The fermentation raw material of chicken manure high-temperature fermentation organic fertilizer is the microbial inoculum, peanut shell, sawdust and brown sugar of the fermented organic fertilizer containing Streptomyces capsularis HWJ001 strain prepared in Example 1, and the fermentation method comprises the following steps,
[0067] a. Preparation of fermentation inoculum, take 500ml of microbial inoculum containing Streptomyces euphorbia HWJ001 strain, add 10L of 5% brown sugar water, and ferment for 6 hours at 37°C on a constant temperature shaker;
[0068] b. Take 5L of the prepared fermented inoculum and spray it evenly on the mixture of peanut shells and sawdust. The mass ratio of peanut shells and sawdust in the mixture is 1:1, and the dosage of the fermented inoculum and the mixture is: fermented bacteria Age...
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Abstract
The invention relates to a thermophilic capsicum actinomycetes and its application, belonging to the technical field of microbes. The sex-tropic actinomycete is Streptomyces capesis HWJ001, which was deposited in the General Microorganism Center of China Committee for Microbial Culture Collection on May 20, 2019, with the preservation number: CGMCC No.17831. The Streptomyces capesis HWJ001 of the present invention can be applied to high-temperature fermentation of bio-organic fertilizers, the fermentation temperature can be as high as 70°C or higher, the fermentation period can be shortened to 18‑21 days, and the fermentation efficiency and the fertilizer efficiency of organic fertilizers can be greatly improved.
Description
technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a thermophilic actinomycete and application thereof. Background technique [0002] Chicken manure is a relatively high-quality organic fertilizer, which contains pure nitrogen, phosphorus (P 2 O 5 ), potassium and other elements. Chicken manure must be decomposed before application, and the parasites and their eggs and other infectious bacteria in the chicken manure are inactivated and deodorized through the decomposed process. It contains medium and trace elements required by plants, active bacteria of beneficial microorganisms and various enzymes. The life activities of these microorganisms in the soil can fix nitrogen in the air, loosen the soil, decompose the phosphorus and potassium minerals in the soil that have been solidified by chemical fertilizers for a long time, and provide enough nutrients for crops. The traditional production technology of bio-org...
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