Long-time chemiluminescence system based on ferriporphyrin metal-organic framework material/glucose oxidase

Abstract

The invention discloses a long-time chemiluminescence system based on ferriporphyrin metal-organic framework material / glucose oxidase. The chemiluminiscence system takes a ferriporphyrin metal-organicframework material / glucose oxidase compound as a catalyst of chemiluminiscence reaction and luminol and glucose as chemiluminiscence substrates, and generates long-time chemiluminiscence by utilizinglocal cascade catalysis of the ferriporphyrin metal-organic framework material and the glucose oxidase. The ferriporphyrin metal-organic framework material is prepared by taking Zr < 4 + > as a metalcenter and ferriporphyrin as a ligand through a hydrothermal method, and has relatively high peroxidase activity and stability; the glucose oxidase is modified on the surface of the ferriporphyrin metal-organic framework material through electrostatic adsorption, and the ferriporphyrin metal-organic framework material / glucose oxidase compound is prepared. A mixed solution system containing the ferriporphyrin metal-organic framework material / glucose oxidase compound, the luminol and the glucose can generate 7.5-hour stable high-intensity chemiluminescence. The chemiluminiscence system can generate long-time stable high-intensity chemiluminiscence under physiological conditions, and has a good application prospect in the aspects of biological detection and imaging.

Description

[0001] 1. Technical field [0002] The invention is a long-time chemiluminescent system based on iron porphyrin metal-organic framework material / glucose oxidase. Using luminol and glucose as chemiluminescent substrates, utilizing the peroxidase activity of iron porphyrin metal-organic framework materials and the localized cascade catalytic properties of nanozyme / biological enzyme complexes to generate long-term stable high-intensity Chemiluminescence, to achieve long-term stable imaging of chemiluminescence. [0003] 2. Background technology [0004] Chemiluminescence is a light radiation phenomenon accompanied by chemical reactions of substances. It has high sensitivity, easy operation, simple instruments and low cost. It is widely used in clinical diagnosis, food testing and biological analysis. Compared with fluorescence methods, chemiluminescence does not require an excitation light source, avoids the interference of autofluorescence, and greatly improves the signal-to-noi...

AI Technical Summary

Problems solved by technology

In addition to biocatalysis, drug delivery, photodynamic therapy and biosensing, metal-organic framework materials have also been used to develop chemiluminescent systems, but the current chemiluminescent systems based on metal-organic framework materials are all flash type, which is not suitable for chemical Luminescence Accurate Detection and Bioimaging Analysis

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