Integrated digital PCR system

Abstract

The invention discloses an integrated digital PCR system, which comprises a digital PCR chip, a position servo driver, a fluorescence image detection device and a temperature circulation device, wherein the temperature circulation device comprises a temperature controller, a heat dissipation module, a TEC fixed on the heat dissipation module and a heat cover arranged above the TEC; an interval height value of the heat cover is greater than a thickness value of the digital PCR chip; the heat cover is a constant-temperature source; and the digital PCR chip is located between the heat cover and the TEC in a temperature circulation process. The digital PCR chip is arranged on the position servo driver, so that the digital PCR chip moves to the fluorescence image detection device from the temperature circulation device. The fluorescence image detection device is suspended and fixed at the upper part of a plane in which the digital PCR chip is located; and the fluorescence image detection device is complementary metal oxide semiconductor imaging principle-based equipment. Therefore, the volume of the equipment is greatly reduced to manufacture portable equipment on the basis of ensuringthe detection efficiency of the digital PCR system, and the applicability to a reagent is improved.

Description

technical field [0001] The present application relates to the technical field of polymerase chain reaction, in particular to an integrated digital PCR system. Background technique [0002] PCR (Polymerase Chain Reaction, Polymerase Chain Reaction) is a molecular biology technique used to amplify and amplify a specific DNA fragment. Single-stranded DNA; when the DNA decomposed into a single strand drops to about 60°C, the single-stranded DNA will bind to the primer; under the action of DNA polymerase, the DNA bound to the primer will undergo semi-conservative replication according to the principle of complementary base pairing. Once the replication is complete, twice as much DNA is available. After multiple cycles of temperature at 95°C and 60°C, a large amount of DNA can be amplified. PCR technology based on the principle of PCR, as the most important molecular biology detection method today, can quickly identify bacteria, viruses, fungi and other microbial types and drug ...

AI Technical Summary

Problems solved by technology

Although TEC is the most widely used temperature cycle method at present, the device can only be used for the matching dPCR chip, and the reaction kit also needs to be customized, which reduces the applicability of some reagents with special formulas. Weak

At the same time, the whole reaction device is relatively bulky

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