A kind of in vitro construction method and application of liver organoids

A construction method and organoid technology, applied in cell dissociation methods, artificial cell constructs, biochemical equipment and methods, etc., to achieve the effects of enhancing proliferation, reducing inflammatory infiltration in the liver, and promoting liver regeneration and repair
CN111394299BActive Publication Date: 2020-12-25NANJING DRUM TOWER HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING DRUM TOWER HOSPITAL
Publication Date
2020-12-25

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Abstract

The invention discloses an in vitro construction method and application of liver organoids. Mouse hepatocytes are extracted to induce the construction of liver organoids, their morphology is observed under a microscope, and their stemness and epithelial cell attributes are identified by immunofluorescence and PCR. Establish a mouse model of acute liver failure after 70% hepatectomy, evaluate the liver function on the 1st, 4th, and 7th day after liver organoid transplantation, compare the liver-to-body ratio, and verify the effect of liver organoids on acute liver failure by HE staining and immunohistochemistry. Therapeutic effects in liver failure. Inflammation and proliferation indicators such as HE staining and ki-67 staining showed that the proliferation of liver organoids was significantly enhanced after transplantation. Liver organoids have strong dryness and proliferation function; they can effectively alleviate the liver function of mice with acute liver failure after 70% hepatectomy, increase the liver-to-body ratio, and promote acute liver failure after 70% hepatectomy by reducing liver inflammation infiltration. Liver regeneration and repair in liver failure.
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Description

technical field

[0001] The invention relates to the technical field of biomedicine, in particular to an in vitro construction method and application of liver organoids. Background technique

[0002] Acute liver failure (ALF) is a clinical syndrome of a large number of liver cell necrosis and severe liver damage in a short period of time caused by factors such as viruses, drugs, toxins, or alcohol, often causing jaundice, hepatic encephalopathy, and multiple organ dysfunction. Complications such as failure and high mortality. Liver transplantation is currently the only effective treatment. However, the shortage of donor livers is far from meeting clinical needs, and many patients died while waiting for liver donors. In addition, medical treatment is only symptomatic and supportive and cannot improve the prognosis of patients, so it is urgent to develop an alternative treatment.

[0003] Stem cells, with their unique capabilities in self-renewal and differentiation potential...

Claims

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