Unlock instant, AI-driven research and patent intelligence for your innovation.

Cell populations comprising cd31-positive, cd45-negative, cd200-positive mammalian cells, and use thereof

A technology of mammals and cell groups, applied to animal cells, vertebrate cells, cells modified by introducing foreign genetic material, etc., to improve blood supply deficiency, low nutrition, and ischemia

Active Publication Date: 2020-07-14
OSAKA UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is known that this cell temporarily differentiates into a vascular endothelial cell-like cell, but cannot continue to contribute as a vascular endothelial cell

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell populations comprising cd31-positive, cd45-negative, cd200-positive mammalian cells, and use thereof
  • Cell populations comprising cd31-positive, cd45-negative, cd200-positive mammalian cells, and use thereof
  • Cell populations comprising cd31-positive, cd45-negative, cd200-positive mammalian cells, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] [Example 1: Identification of mouse liver vascular endothelial stem cells based on cell surface markers]

[0108] Using the SP cell population and the MP cell population obtained from the vascular endothelial cells (CD31-positive CD45-negative cells) of the mouse liver, comprehensively analyzed the genes expressed more in the SP cell population than in the MP cell population, and found out More than 100 genes. From these genes an attempt was made to identify cell surface markers of vascular endothelial stem cells present in about 10% of the SP cell population.

[0109] 1-1 Analysis of surface markers of mouse liver vascular endothelial cells

[0110] (1) Made using animals and cells

[0111] C57BL / 6 mice and C57BL / 6-Tg(CAG-EGFP) mice (commonly known as green mice) were purchased from Japan SLC, Inc. Mice aged 8 to 12 weeks were used in the experiments. The mice were laparotomed under anesthesia, and the liver was removed. Cut the liver into pieces, immerse in a m...

Embodiment 2

[0132] [Example 2: Treatment of hemophilia based on transplantation of CD157-positive CD200-positive vascular endothelial cells]

[0133] Since stem cells can continue to generate terminally differentiated somatic cells while maintaining their undifferentiated nature, transplantation of CD157-positive and CD200-positive cells, which are considered to be vascular endothelial stem cells, can be expected to continue contributing as vascular endothelial cells for a long time. Therefore, it is considered that transplantation of these cells to a patient suffering from a disease caused by dysfunction of vascular endothelial cells may potentially cure the disease. Therefore, it was investigated whether transplantation of normal CD157-positive CD200-positive cells into the liver of hemophilia A model mice could suppress the bleeding tendency of hemophilia A model mice.

[0134] (1) Experimental method

[0135] Hemophilia A model mice (coagulation factor VIII gene-deficient mice) were ...

Embodiment 3

[0142] [Example 3: Liver regeneration based on transplantation of CD157-positive CD200-positive vascular endothelial cells]

[0143] In recent years, it has been reported that molecules secreted by vascular endothelial cells play an important role in maintaining tissue homeostasis or tissue remodeling. For the liver, the maintenance mechanism of liver cells guided by vascular endothelial cells in hepatic sinusoidal vessels has also been elucidated. . Since it is known that CD157-positive CD200-positive vascular endothelial cells contribute significantly to the formation of hepatic sinusoidal vessels, it was investigated whether transplantation of CD157-positive CD200-positive vascular endothelial cells can promote liver regeneration.

[0144] 3-1 Liver regeneration experiment 1

[0145] (1) Experimental method

[0146] 70% partial hepatectomy was performed on C57BL / 6 mice according to conventional methods. According to the method described in 1-1 of Example 1, CD157-positiv...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

Provided are: a cell population which is essentially formed from mammalian cells in which cell surface markers CD31 and CD200 are positive, and cell surface marker CD45 is negative; a cell populationwhich is essentially formed from mammalian cells in which cell surface markers CD31, CD157, and CD200 are positive, and cell surface marker CD45 is negative; mammalian vascular endothelial stem cellsin which cell surface marker CD31 is positive, cell surface marker CD157 and / or cell surface marker CD200 are / is positive, and cell surface marker CD45 is negative; and a revascularizing pharmaceutical having, as an active ingredient, the cell populations or the vascular endothelial stem cells.

Description

technical field [0001] The present invention relates to a cell group composed of CD31-positive, CD45-negative, CD200-positive mammalian cells, and a medicament using the cell group as an active ingredient. Background technique [0002] Hematopoietic stem cells present in the bone marrow are known to maintain bone marrow hematopoiesis for a long time by the ability to divide in an undifferentiated state (self-replicating ability) and the ability to differentiate into progenitor cells that generate red blood cells as mature blood cells or white blood cells, platelets. On the other hand, it has been considered that vascular endothelial cells forming the inner wall of blood vessels are generated in the fetal period as mature vascular endothelial cells and maintain blood vessels throughout life by slowly dividing. That is, it does not take into account the presence of stem cells in the vascular system that support blood vessels for a long time. [0003] The inventors of the pre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C12N5/10A61K35/44A61P3/06A61P3/10A61P7/04A61P3/02A61P9/00A61P9/10A61P9/12A61P19/10C12Q1/02
CPCA61K35/44A61P3/02A61P3/06A61P3/10A61P7/04A61P9/00A61P9/10A61P9/12A61P19/10A61P3/00C12N5/0692G01N33/5073G01N33/5044G01N33/5014A61P1/16C12N2509/10
Inventor 高仓伸幸内藤尚道若林卓
Owner OSAKA UNIV