Use of a willow orchid leaf plant extract in the preparation of products for inhibiting the germination of lettuce seeds
A technology of plant extract and lettuce seeds, which is applied in the directions of botanical equipment and methods, chemicals for biological control, plant growth regulators, etc., and can solve the problems of no allelopathy of willow orchid
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Embodiment 1
[0017] Embodiment 1 Preparation of water extracts from different parts of Willow Orchid
[0018] The donor plant willow orchid is harvested during the flowering stage, divided into four different parts: flowers, stems, capsules and leaves, dried naturally in the shade, crushed into fine powder, and passed through a 100-mesh sieve. Take 10g of flower, stem, capsule and leaf powder of different parts of Willow Orchid, add 100mL of water, shake and extract for 24 hours, the extraction temperature is 30°C, filter, take the filtrate and add water to 100mL, and get 100mg / mL plant extract of different parts of Willow Orchid ; Dilute 100mg / mL plant extract 3 times to get 33mg / mL plant extract; Dilute 100mg / mL plant extract 10 times to get 10mg / mL plant extract; Dilute 100mg / mL plant extract 33 times to get 3mg / mL mL plant extract; dilute 100mg / mL plant extract 100 times to obtain 1mg / mL plant extract.
Embodiment 2
[0019] Embodiment 2 lettuce seed germination assay
[0020] Select lettuce seeds with full grains, uniform size, and no pests and diseases as recipient plants, sterilize with 10% sodium hypochlorite for 10 minutes, rinse with distilled water for 5 times, and dry for later use. Petri dish filter paper method was used. Double-layer filter paper was placed on the bottom of the petri dish, and after high-temperature sterilization, 5 mL of water extracts from different parts of Willow Orchid with a mass concentration of 100 mg / mL, 33 mg / mL, 10 mg / mL, 3 mg / mL and 1 mg / mL were added respectively, and no Bacteria distilled water 5mL was used as a control, and 30 lettuce seeds were placed in each petri dish respectively, and three parallels were set up in different concentration groups respectively, and the petri dish was placed in an intelligent artificial incubator (the light intensity was 3000lx, the light time was 12 hours, and the temperature was 20±1° C., humidity 75% RH) for 48...
Embodiment 3
[0025] Embodiment 3 The influence of the water extract of different parts of Willow Orchid on the germination rate of lettuce seeds
[0026]Adopt the method described in embodiment 2 to measure. The results are shown in Table 1 and Table 2. The results showed that the treatment groups of 1mg / mL and 3mg / mL water extracts of different parts of Willow Orchid, 10mg / mL and 33mg / mL of water extracts of Willow Orchid flowers, stems and capsules had no significant effect on the germination rate of lettuce seeds (P>0.05) . And the 100mg / mL treatment group of water extracts from different parts had a very significant inhibitory effect on the germination of lettuce seeds (Pthe inhibitory effect of capsule water extract>the inhibitory effect of flower water extract>stem water The inhibitory effect of the extract, RI were -1.000, -0.976, -0.955 and -0.636, respectively.
[0027] Table 1 Effects of extracts from different parts of Willow Orchid on the germination rate of lettuce seeds
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