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Method for establishing iodine absorption inhibition rat model

A method for establishing a rat model, which is applied in the field of biomedicine, can solve the problems of not being able to simulate the natural course of the disease well, long experimental cycle, time-consuming and labor-consuming, etc., and achieve the effect of short experimental cycle, high success rate, and low cost

Inactive Publication Date: 2020-07-28
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The above-mentioned rat model of iodine deficiency disease established by feeding low-iodine feed can better replicate the natural occurrence and development process of human diseases and obtain valuable research data, but the experiment cycle is relatively long and consumes more manpower, financial and material resources
The rat hypothyroidism model induced by giving antithyroid drugs, such as PTU and MTU, is relatively simple and has a high success rate, but it still has the disadvantages of relatively long experimental period, time-consuming and labor-intensive, and the inability to simulate the natural course of the disease well. Not strictly an animal model of iodine deficiency

Method used

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  • Method for establishing iodine absorption inhibition rat model
  • Method for establishing iodine absorption inhibition rat model
  • Method for establishing iodine absorption inhibition rat model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Rat model establishment

[0038] Use corn oil as a solvent to prepare zinc thiazole suspensions with concentrations of 8 mg / kg.bw, 16 mg / kg.bw, and 40 mg / kg.bw, respectively.

[0039] 32 SD rats were randomly divided into 4 groups: corn oil solvent control group, 8mg / kg.bw exposure group, 16mg / kg.bw exposure group and thiazole zinc 40mg / kg.bw exposure group, once a day, continuous Gastrointestinal poisoning for 6 days.

[0040] 2. Identification

[0041] 24 hours after the end of exposure, the blood of the rats was collected, serum was prepared, and the levels of TT3, TT4 and TSH were detected; the thyroid was weighed and the thyroid histopathological examination was performed.

[0042] 3. Results

[0043]Compared with the control group, thiazole zinc suspension at 16mg / kg.bw and 40mg / kg.bw doses, exposed rats for 6 days, significantly caused rat thyroid weight to increase ( figure 1 ); causing the thyroid follicular epithelium to be cubic or tall columnar, with ...

Embodiment 2

[0045] 1. Rat model establishment

[0046] Use corn oil as a solvent to prepare zinc thiazole suspensions with a concentration of 8 mg / kg.bw and 40 mg / kg.bw, respectively.

[0047] 30 SD rats were randomly divided into 5 groups: corn oil solvent control group, thiazole zinc 8mg / kg.bw exposure group (6 days exposure), thiazole zinc 40mg / kg.bw exposure group (6 days exposure) , Zinc thiazole 40mg / kg.bw poisoning group (1 day of poisoning), Zinc thiazole 40mg / kg.bw poisoning group (3 days of poisoning), once a day, intragastric poisoning.

[0048] 2. Identification

[0049] After the last exposure, the rats were injected with 185kBp[125I]-NaI through the tail vein. After 24 hours, the rats were sacrificed, the thyroid gland was separated and weighed, and the absorption of radioactive iodine by the thyroid gland was measured with a gamma counter.

[0050] 3. Results

[0051] The results of radioactive iodine absorption experiments in rats showed that zinc thiazole at doses of 8...

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Abstract

The invention belongs to the technical field of biological medicines, and relates to a method for establishing an iodine absorption inhibition rat model. The establishment method comprises the following steps: preparing a zinc thiazole suspension; carrying out one-time gavage or more than two-time gavage on a rat by utilizing the zinc thiazole suspension, so as to establish the iodine absorption inhibition rat model; and during more than two times of gavage, the repeated time interval of two times is 18-36 hours. The method for establishing the iodine absorption inhibition rat model is simpleto operate, low in cost, easy to repeat, short in experimental period and high in success rate.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically relates to a method for establishing a rat model of iodine absorption inhibition. Background technique [0002] As a regulator of growth and development, metabolism, cardiovascular function and nervous system, thyroid hormones participate in various physiological processes of the body. Thyroid hormones are critical to the nervous system and skeletal development of infants, especially during the first four months of life. Lack of thyroid hormone during the fetal period and early postnatal period will greatly affect the growth and maturation of the brain, resulting in hypoplasia of the brain, resulting in cretinism with mental, nervous and bone developmental disorders as the main manifestations. Iodine is an essential raw material for the synthesis of thyroid hormones. Iodine deficiency or inhibition of iodine absorption leads to the reduction of thyroid hormones in the b...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/20A01K2227/105A01K2267/03
Inventor 杨红莲刘焕亮崔博林本成杲修杰王坤佘晓俊马科峰张伟
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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