Biopesticide for preventing and treating downy mildew
A bio-pesticide and downy mildew technology, applied in biocides, chemicals for biological control, biocides, etc., can solve problems such as affecting the field operation of orchards, affecting the growth of fruit trees and saplings, and requiring large amounts of fertilizer and water. , to achieve the effect of reducing bacterial resistance, difficult drug resistance, and reducing pesticide environmental pollution
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Embodiment 1
[0018] indoor bioassay
[0019] test target
[0020] Grape downy mildew (Plasmopara viticola), strains collected from the field.
[0021] Test material preparation
[0022] Select the susceptible grape variety Gembo, from top to bottom 4 to 6 leaf positions, cut the leaves with 1cm to 2cm petiole in the same part, the same growth, wrap the petiole with wet cotton balls and place it in a petri dish, keep it moist for later use .
[0023] Test drug
[0024] 98% eugenol, product of Jiangsu Jintan Weidelong Chemical Co., Ltd.; 31% ethanol dry crude extract of wheat straw and eugenol compound fungicide, prepared by Zaozhuang College.
[0025] Control drug
[0026] 98% dimethomorph technical product, product of Shenyang Fengshou Pesticide Co., Ltd., Liaoning Province.
[0027] experiment procedure
[0028] S1. Preparation of sporangia suspension
[0029] Select the natural diseased leaves in the field, wash the downy mildew sporangia on the back of the leaves with 4°C distil...
Embodiment 2
[0061] According to the screened optimal compounding ratio of ethanol crude extract of wheat straw and eugenol 30:1, a compound fungicide of 31% ethanol crude extract of small wheat straw and eugenol was prepared, which contained 30% wheat straw ethanol crude extract and 1% eugenol. In the field, 30% wheat straw ethanol crude extract WP 800 times, 0.3% eugenol SL 800 times tank mix, and 50% dimethomorph WP 1500 times were used as control agents to carry out field efficacy tests.
[0062] The test object is downy mildew.
[0063] The experimental crop is grapes, and the experimental grape varieties are selected from gems.
[0064] environmental conditions
[0065] The hedgerow cultivation in the experimental park is 9 years old, and the occurrence of downy mildew is common and uniform over the years. During the test period (August 1-August 30) the grape growth period to the new shoot growth period. The cultivation conditions and tree growth of all experimental plots were co...
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