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Method for separating sporophyll mucus of undaria pinnatifida

A technology of undaria spore leaf and separation method, which is applied in the field of natural product separation, can solve the problems of high cost and large amount of enzyme, achieve the effect of stabilizing active components and avoiding greening of algae

Active Publication Date: 2020-07-28
DALIAN HAIBAO FISHERY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adopting the method of the present invention can make the sporophyll mucus of Undaria pinnata secrete from the inside of the sporophyll and separate from the surface of the sporophyll, so that fucoidan can be extracted from the sporophyll mucus of Undaria pinnatifida, and solve the problem of directly extracting fucoidan from the sporophyll of Undaria pinnata. The problem of large amount of enzymes and high cost in extracting fucoidan, alginate and other biologically active substances from

Method used

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  • Method for separating sporophyll mucus of undaria pinnatifida
  • Method for separating sporophyll mucus of undaria pinnatifida
  • Method for separating sporophyll mucus of undaria pinnatifida

Examples

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Effect test

Embodiment 1

[0043] Get 500g of fresh wakame sporophylls collected in the middle of the harvest period, put them into a 20L plastic bucket, add 10L of fresh water to the plastic bucket, stir and wash for 3 minutes;

[0044] Take out the sporophyll, dry in the shade under controlled water for 60 minutes under 20LUX light intensity, the mucus is released from the inside of the sporophyll to the surface of the sporophyll;

[0045] Prepare 1L of 0.025M NaOH solution, 0.01M NaOH 2 CO 3 1L of the solution was poured into a 10L polyethylene bucket and mixed evenly to obtain mucus separation liquid;

[0046] Soak the sporophyll after drying in the shade for 1 hour in the mucus separation liquid for 30 minutes, the algae should not be exposed to the liquid surface, and stir once every 5 minutes.

[0047] After soaking, adjust the pH of the mucus separation liquid to 6.0 with 1M hydrochloric acid solution; remove the wakame spore leaves from the mucus separation liquid after stirring for 5 minutes...

Embodiment 2

[0050] Get 1000g of fresh wakame sporophylls collected in the later stage of the harvest period, put them into a 20L plastic bucket, add 10L of fresh water, stir and wash for 4 minutes;

[0051] Take out the sporophyll, dry in the shade under water control for 60 minutes, the mucus is released from the inside of the sporophyll to the surface of the sporophyll;

[0052] Prepare 2L of 0.05M NaOH solution, 0.05M NaOH 2 CO 3 2L of the solution was poured into a 10L polyethylene bucket and mixed evenly to obtain mucus separation liquid;

[0053] Soak the sporophyll after drying in the shade for 1 hour in the mucus separation liquid for 60 minutes, the algae should not be exposed to the liquid surface, and stir once every 5 minutes.

[0054] After soaking, adjust the pH of the mucus separation liquid to 6.0 with 1M hydrochloric acid solution; pull out the wakame sporophylls from the mucus separation liquid after stirring for 5 minutes, and the wakame sporophyll mucus stays in the ...

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Abstract

The invention discloses a separation method of sporophyll mucus of undaria pinnatifida. The separation method comprises the following steps: (1) putting fresh or unfrozen chilled sporophyll of undariapinnatifida into fresh water for cleaning; and (2) taking out the washed undaria pinnatifida sporophyll, drying the undaria pinnatifida sporophyll in the shade for 0.5-3 hours, soaking the undaria pinnatifida sporophyll in mucus separation liquid for 20-60 minutes, adjusting the pH value of the mucus separation liquid to 5.0-6.5 with hydrochloric acid after soaking is finished, and taking out theundaria pinnatifida sporophyll after stirring to obtain the sporophyll mucus of undaria pinnatifida, wherein the mucus separation liquid is formed by mixing a sodium hydroxide solution and a sodium carbonate solution. By adopting the method disclosed by the invention, the sporophyll mucus of undaria pinnatifida can be effectively separated from the undaria pinnatifida sporophyll, so fucoidin, sodium alginate and the like can be extracted by utilizing the sporophyll mucus of undaria pinnatifida, and the problems of high enzyme consumption and high cost in directly extracting the fucoidin and the like from the undaria pinnatifida sporophyll are solved.

Description

technical field [0001] The invention relates to the technical field of natural product separation, in particular to a method for separating the mucus of undaria spore leaves. Background technique [0002] Undaria pinnata sporophyll is the reproductive organ of the large marine brown algae Undaria pinnatae, located at the base of the stem of the seaweed, shaped like fungus, commonly known as "ears". Since the wakame spore leaves are rich in precious marine bioactive substances such as fucoidan and alginate, and the quality of the active substances is excellent, the wakame spore leaves become an important raw material for extracting fucoidan and alginate. [0003] Fucoidan (fucoidan), also known as fucoidan sulfate, has significant anti-tumor effects, and also has various biological activities such as anti-coagulation, lowering blood lipids, anti-virus, and enhancing the immune function of the body. Fucoidan powder and beverages containing fucoidan have been sold in domestic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C08B37/04
CPCC08B37/0063C08B37/0084C08B37/0003
Inventor 张喜昌何云海曹淑青邹品
Owner DALIAN HAIBAO FISHERY
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