Solid-state nanopore mechanism-based single molecule detection method and system

A single-molecule detection and nanopore technology, applied in the biological field, to achieve the effects of long-read sequencing performance, high controllability, and high-precision sequencing performance

Pending Publication Date: 2020-07-28
苏州罗岛纳米科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the magnetic field range of these magnetic tweezers systems is too large, which often causes the movement of a large number of magnetic beads, and at the same time, the movement of a large number of DNA attached to the magnetic beads makes the nanopore no longer a single molecule.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Solid-state nanopore mechanism-based single molecule detection method and system
  • Solid-state nanopore mechanism-based single molecule detection method and system
  • Solid-state nanopore mechanism-based single molecule detection method and system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] The specific implementation manners of the present invention will be described below in conjunction with the accompanying drawings.

[0038] The present invention provides a single-molecule detection method based on a solid-state nanopore mechanism:

[0039] like figure 1 As shown, the solid-state nanopore mechanism includes a nanopore component 1 , an upper liquid pool 2 and a lower liquid pool 3 .

[0040] The nanopore component 1 is arranged at the bottom of the upper liquid pool 2, and the nanopore component 1 has nanopores 11 running through both sides thereof. Nanopore components can be semiconductor chips or made of other materials. The upper liquid pool 2 and the lower liquid pool 3 hold liquids with conductive properties respectively. The nanopore part 1 (the bottom of the upper liquid pool 2) is immersed in the lower liquid pool 3, the liquid in the upper liquid pool 2 and the lower liquid pool 3 is isolated by the nanopore part 1, and the lower liquid pool...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a solid-state nanopore mechanism-based single molecule detection method and system. A solid-state nanopore mechanism includes a nanopore component; the nanopore component is provided with nanopores penetrating through two sides of the nanopore component, and the two sides of the nanopore component are each provided with liquid having a conductive property; and the liquid atthe two sides can be equipped with a substrate and magnetic beads, respectively. The single molecule detection method comprises the following steps: a to-be-detected single molecule chain and the substrate and the magnetic beads undergo biological modification, separately; one end of the single molecule chain is linked to the substrate of the liquid at one side; after passing through the nanopores, the other end of the single molecule chain is linked with the magnetic beads of the liquid at the other side; the single molecule chain is stretched and fixed under the action of magnetic tweezersto control the single molecule chain and the nanopore to oppositely move to obtain a detection signal, and accordingly, biochemical characteristics of single molecules are detected, such as DNA sequences. The method and the system have the advantages of long read length, high controllability and high precision.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a single-molecule detection method, in particular to a single-molecule chain detection method and system based on a solid-state nanopore mechanism, which can be applied to gene sequencing and other single-molecule biological and chemical characteristic detection. Background technique [0002] Gene sequencing technology is an important technical means of life sciences and an indispensable tool for molecular diagnosis and biomedicine. Medical application services are the fastest growing and most potential part of gene sequencing technology, involving prenatal screening, tumor diagnosis, genetic disease diagnosis, preimplantation diagnosis, etc. At present, according to different principles, people divide the development of sequencing technology into three stages, the first generation, Sanger sequencing. The second generation, high-throughput sequencing (NGS). Third generation, single mo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6869C12M1/42C12M1/36C12M1/34
CPCC12Q1/6869C12Q2563/143C12Q2563/155C12Q2565/631
Inventor 王维平吴宏文胡岚凌新生
Owner 苏州罗岛纳米科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap