Solid-state nanopore mechanism-based single molecule detection method and system

Abstract

The invention discloses a solid-state nanopore mechanism-based single molecule detection method and system. A solid-state nanopore mechanism includes a nanopore component; the nanopore component is provided with nanopores penetrating through two sides of the nanopore component, and the two sides of the nanopore component are each provided with liquid having a conductive property; and the liquid atthe two sides can be equipped with a substrate and magnetic beads, respectively. The single molecule detection method comprises the following steps: a to-be-detected single molecule chain and the substrate and the magnetic beads undergo biological modification, separately; one end of the single molecule chain is linked to the substrate of the liquid at one side; after passing through the nanopores, the other end of the single molecule chain is linked with the magnetic beads of the liquid at the other side; the single molecule chain is stretched and fixed under the action of magnetic tweezersto control the single molecule chain and the nanopore to oppositely move to obtain a detection signal, and accordingly, biochemical characteristics of single molecules are detected, such as DNA sequences. The method and the system have the advantages of long read length, high controllability and high precision.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a single-molecule detection method, in particular to a single-molecule chain detection method and system based on a solid-state nanopore mechanism, which can be applied to gene sequencing and other single-molecule biological and chemical characteristic detection. Background technique [0002] Gene sequencing technology is an important technical means of life sciences and an indispensable tool for molecular diagnosis and biomedicine. Medical application services are the fastest growing and most potential part of gene sequencing technology, involving prenatal screening, tumor diagnosis, genetic disease diagnosis, preimplantation diagnosis, etc. At present, according to different principles, people divide the development of sequencing technology into three stages, the first generation, Sanger sequencing. The second generation, high-throughput sequencing (NGS). Third generation, single mo...

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Problems solved by technology

However, the magnetic field range of these magnetic tweezers systems is too large, which often causes the movement of a large number of magnetic beads, and at the same time, the movement of a large number of DNA attached to the magnetic beads makes the nanopore no longer a single molecule.

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