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Live vaccine for treating swine enzootic pneumonia and preparation method of live vaccine

A technology for swine asthma and live vaccine, which is applied in biochemical equipment and methods, medical preparations containing active ingredients, viruses, etc., can solve the problems of unstable price of adjuvant, unsatisfactory immune effect, and difficulty in popularization and use. Achieve the effect of reducing the probability of adverse reactions, low cost, and strong antigen stability

Pending Publication Date: 2020-07-31
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because these adjuvants are unstable and expensive, it is difficult for domestic vaccine manufacturers to popularize and use them.
Dilute the vaccine with deionized water or normal saline or PBS buffer solution to inoculate animals, often resulting in unsatisfactory immune effect or immune failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A live vaccine for treating swine asthma. The inactivated vaccine contains inactivated mycoplasma suis strains and an adjuvant allowed for use in veterinary biological products. The inactivated bacteria are used as the water phase, the adjuvant as the oil phase, and the water phase It is emulsified with the oil phase in proportion, and the water phase and the oil phase are emulsified according to the volume ratio of 1:1~1:3.

[0036] Further improved, the concentration of the virus liquid in the vaccine is 1.05×107EID50 / 0.1ml, and the chitosan is 400mg.

[0037] Further improved, its preparation method for the live vaccine for the treatment of porcine asthma comprises the steps:

[0038] S1: Unpack the freeze-dried strains, inoculate the strains in liquid M199 medium at 12% inoculum, cultivate at 30-35.5°C for 3-7 days, and harvest the strains when the pH of the medium is 6.8 And conduct a pure inspection, mark the harvest date, strain generation, and after the inspect...

Embodiment 2

[0049] A live vaccine for treating swine asthma. The inactivated vaccine contains inactivated mycoplasma suis strains and an adjuvant allowed for use in veterinary biological products. The inactivated bacteria are used as the water phase, the adjuvant as the oil phase, and the water phase It is emulsified with the oil phase in proportion, and the water phase and the oil phase are emulsified according to the volume ratio of 1:3.

[0050] Further improved, the concentration of the virus liquid in the vaccine is 1.05×107EID50 / 0.1ml, and the chitosan is 400mg.

[0051] Further improved, its preparation method for the live vaccine for the treatment of porcine asthma comprises the steps:

[0052] S1: Unpack the freeze-dried strains, inoculate the strains in liquid M199 medium at 15% inoculum size, culture at 35.5°C for 7 days, and when the pH value of the medium is 5.8, harvest the strains and conduct a pure test , mark the date of harvest, the generation of the strain, and after t...

Embodiment 3

[0063] A live vaccine for treating swine asthma. The inactivated vaccine contains inactivated mycoplasma suis strains and an adjuvant allowed for use in veterinary biological products. The inactivated bacteria are used as the water phase, the adjuvant as the oil phase, and the water phase It is emulsified with the oil phase in proportion, and the water phase and the oil phase are emulsified according to the volume ratio of 1:1.

[0064] Further improved, the concentration of the virus liquid in the vaccine is 1.05×107EID50 / 0.1ml, and the chitosan is 400mg.

[0065] Further improved, its preparation method for the live vaccine for the treatment of porcine asthma comprises the steps:

[0066] S1: Unpack the freeze-dried strains, inoculate the strains in liquid M199 medium at 12% inoculum, culture at 30°C for 3 days, and when the pH value of the medium is 6.8, harvest the strains and perform a pure test , mark the date of harvest, the generation of the strain, and after the insp...

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PUM

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Abstract

The invention discloses a live vaccine for treating swine enzootic pneumonia. The live vaccine is characterized in that the inactivated vaccine contains inactivated porcine mycoplasmas strains and adjuvants allowed by veterinary biological products, the inactivated strains are used as a water phase and the adjuvants are used as an oil phase, and the water phase and the oil phase are emulsified according to a volume ratio of 1: 1-1: 3. The prepared vaccine is high in antigen stability, high in immunogenicity, high in antigen recovery rate, high in vaccine safety, capable of reducing the probability of adverse reactions and improving the immune effect, simple in preparation method and low in cost. Therefore, the live vaccine for treating the swine enzootic pneumonia provided by the inventionhas a wide market prospect.

Description

technical field [0001] The invention relates to the technical field of urticaria treatment, in particular to a live vaccine for treating swine asthma and a preparation method thereof. Background technique [0002] Mycoplasma swine pneumonia, commonly known as swine asthma, is a chronic respiratory infectious disease of pigs caused by Mycoplasma hyopneumoniae. The main symptoms are cough and wheezing. The disease mainly causes the feed conversion rate and growth retardation of pigs, with high morbidity and low mortality. Mycoplasma suis pneumonia is widely prevalent in the world, causing significant economic losses to the modern pig industry. Mycoplasma swine pneumonia is a chronic intractable infectious disease. The effect of drug treatment is not satisfactory, and it is easy to relapse after stopping the drug. Once the disease is prevalent in pig farms, it is difficult to eradicate it. Therefore, the prevention, control and purification of mycoplasma pneumonia must take c...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K39/39A61P11/00A61P11/06A61P11/14A61P31/04A61K39/12A61P31/20
CPCA61K39/0241A61K39/12A61K39/39A61K2039/521A61K2039/5252A61P11/00A61P11/06A61P11/14A61P31/04A61P31/20C12N2750/10034
Inventor 倪博袁朗
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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