PCR primer group and kit for detecting JAK2V617F and CALR ninth exon gene mutation

Abstract

The invention belongs to the technical field of gene detection, and in particular relates to a PCR primer group and kit for detecting JAK2V617F and CALR ninth exon gene mutation. Multiple PCR and a genetic analysis system based on a capillary electrophoresis technology are combined, and JAK2V617F and CALR ninth exon gene mutation detection is completed at the same time in one PCR reaction system.The PCR product fragment analyzed by the capillary electrophoresis fragment analysis method has high resolution, so that the PCR product fragment can be identified even if non-specific amplification occurs, and the traditional means can be interpreted to be positive only when a fluorescence signal occurs at a site with a specific fragment size. The PCR primer group and kit have high sensitivity, and can recognize mutations in DNA minimum to 0.1 ng / [mu]L and mutation of at least 0.1%. The flux is high, and the method can be used for simultaneously analyzing the gene mutation conditions of 96 specimens, and can be applied to large-scale disease screening.

Description

technical field [0001] The invention belongs to the technical field of gene detection, in particular to a PCR primer set and a kit for detecting JAK2 V617F and CALR exon nine gene mutations. Background technique [0002] BCR-ABL negative myeloproliferative neoplasms (myeloproliferative neoplasms, MPN), polycythemia vera (PV), essential thrombocythemia (essential thrombocythemia, ET) and primary myelofibrosis (primary myelofibrosis, PMF) ) and other diseases. Such diseases are mainly caused by mutations in normal human genes, resulting in the active proliferation of one or more lines of bone marrow cells. Therefore, the discovery and detection of gene mutations has become the main diagnostic method for MPN. Recent studies have shown that JAK2 (Janus kinase 2), CALR (calreticulin), MPL (myeloproliferative leukemia virus gene) and other gene mutations mainly exist in MPN patients, and JAK2, CALR, and MPL gene mutations have become the main criteria for the diagnosis of MPN. ...

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Problems solved by technology

However, there are no related reports at home and abroad that use the fragment analysis function of the genetic analysis system based on capillary electrophoresis technology to simultaneously detect the deletion or insertion mutation of exon 9 of JAK2V617F and CALR.

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