Human umbilical cord mesenchymal stem cell injection, preparation method and application thereof

A technology of mesenchymal stem cells and human umbilical cord, applied in the field of stem cells, can solve problems such as aggravating the bleeding risk of patients with liver cirrhosis, and achieve favorable and good therapeutic effects

Pending Publication Date: 2020-08-07
SHANGHAI CRYOWISE MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, low anticoagulant heparin is a component used to prevent blood clot formation, and patients with liver cirrhosis lack coagulation factors and have coagulation dysfunction due to impaired liver function, and the application of low anticoagulant heparin will increase the risk of bleeding in patients with liver cirrhosis

Method used

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  • Human umbilical cord mesenchymal stem cell injection, preparation method and application thereof
  • Human umbilical cord mesenchymal stem cell injection, preparation method and application thereof
  • Human umbilical cord mesenchymal stem cell injection, preparation method and application thereof

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Embodiment Construction

[0031] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. the embodiment. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein shall have the usual meanings understood by those skilled in the art to which the present invention belongs. As used herein, "comprising" and similar words mean that the elements or items appearing before the word include the elements or items listed after the word and their equivalents, without excluding other elements or items.

[0032] ...

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Abstract

The invention provides a human umbilical cord mesenchymal stem cell injection, which is composed of human umbilical cord mesenchymal stem cells and a cryopreservation liquid. The cryopreservation liquid is composed of human serum albumin, clinical dimethyl sulfoxide and a compound electrolyte injection. The living cell density of the human umbilical cord mesenchymal stem cell injection is controlled at 0.5*10<6>-2*10<6>/ml, so that the cell viability of the human umbilical cord mesenchymal stem cell injection after cryopreservation and resuscitation can still reach 90% or above, and the treatment effect is facilitated; the content of the human albumin and the content of the clinical dimethyl sulfoxide are controlled to be 3-20% and 5-10% respectively, so that the human umbilical cord mesenchymal stem cell injection can be directly used for injection, can still be safely used after being cryopreserved for at least 72 hours, and is beneficial to achievement of a good treatment effect. The invention also provides a preparation method of the human umbilical cord mesenchymal stem cell injection and application of the human umbilical cord mesenchymal stem cell injection in treatment of liver failure.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a human umbilical cord mesenchymal stem cell injection, a preparation method and an application thereof. Background technique [0002] Liver failure is severe liver damage caused by a variety of factors, leading to serious impairment of liver synthesis, metabolism, detoxification and biotransformation, causing a large amount of toxic substances to accumulate in the body, causing coagulation dysfunction, hyperbilirubinemia, and hepatic encephalopathy It is a group of clinical syndromes mainly manifested by ascites and ascites, which is difficult to treat and has a very high case fatality rate. [0003] Compared with orthotopic liver transplantation, hepatic cell transplantation has gradually become an emerging method for the treatment of liver diseases due to its advantages of safety, reliability, low immunogenicity, wide range of sources, less difficult operation, and lower tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/10A61K47/42A61P1/16
CPCA61K9/0019A61K9/10A61K35/28A61K47/42A61P1/16
Inventor 朱雪晶鄢和新
Owner SHANGHAI CRYOWISE MEDICAL TECH CO LTD
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