Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for diagnosing age-related macular degeneration by measuring peripheral blood CRX genes

A macular degeneration and correlation technology, applied in the field of biomedicine, can solve problems such as visual function damage, fundus pathological changes, and limited treatment effect of patients

Pending Publication Date: 2020-08-07
于复东 +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the visual function of AMD found in imaging examinations is often damaged, and typical fundus pathological changes appear, the treatment effect of patients is limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for diagnosing age-related macular degeneration by measuring peripheral blood CRX genes
  • Method for diagnosing age-related macular degeneration by measuring peripheral blood CRX genes
  • Method for diagnosing age-related macular degeneration by measuring peripheral blood CRX genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Example 1 CRX gene expression analysis

[0126] From the gene expression data set of AMD patient tissues in Iowa, a total of 83 samples (including 42 normal groups, 16 pre-AMD groups, 16 dry-AMD groups, and 9 wet-AMD groups) were obtained through the screening of redundant samples. (Cy5 / Cy3) expression data, and the analysis of differentially expressed genes (t test).

[0127] see results Figure 1-3 , figure 1 The gene expression of CRX in the tissue samples of this study is shown; figure 2 Confidence intervals between CRX gene expression groups in the tissue samples of this study are shown; image 3 The statistical results of the CRX gene expression group in the tissue samples of this study are shown. The CRX gene is differentially down-regulated in the AMD patient group and the normal group (P. value=0.029, fold Change value=-1.177), and in the wet-AMD group and the normal group The expression was differentially down-regulated in the normal group (P. value = 0.0...

Embodiment 2

[0128] Example 2 methylation analysis results

[0129] Corresponding gene expression in retinal or macular retinal pigment epithelium-choroidal tissue from AMD patients to gene expression signatures in blood samples was validated on the methylation dataset in blood tissue samples from John Hopkins University. In the methylation expression data set of John Hopkins University (nine in the AMD group and nine in the normal control group), the eBayes model was used for statistical analysis through de-redundancy and data preprocessing.

[0130] see results Figure 4 . Figure 4 It shows the results of CRX methylation in the blood samples of this study. The promoter region of the CRX gene is hypermethylated, among which TSS200, TSS1500, 1stExon and 5'UTR, 3'UTR, and 5'UTR are differentially hypermethylated , respectively TSS200 (P. value = 0.0173, fold Change value = 0.027), TSS1500 (P. value = 0.0324, fold Change value = 0.0603), 1stExon and 5'UTR (P. value = 0.0142, fold Change v...

Embodiment 3

[0131] Preparation and verification of embodiment 3 kit

[0132] This embodiment provides a kit for detecting the expression of CRX gene. This kit can detect the expression level of CRX gene:

[0133] Kit main reagents:

[0134] (1) Amplification primers: upstream primer (F) and downstream primer (R);

[0135] (2) Main reagents for PCR: Pfu high-fidelity enzyme, 10×PCR Buffer, dNTPMixtur, ddH 2 O;

[0136] (3) Main reagents for gene expression level detection; and / or

[0137] (4) Main reagents for methylation detection.

[0138] The invention has also been tested and validated in a population sample. The results show that the expression level and / or methylation of CRX gene can provide useful auxiliary diagnostic information for age-related macular degeneration, especially for early and / or auxiliary diagnosis.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for diagnosing age-related macular degeneration by measuring peripheral blood CRX genes. Specifically, the invention provides the application of a CRX gene or a locus thereof and / or a detection reagent thereof to preparation of a reagent or a kit, and the reagent or the kit is used for evaluating the illness risk of an object to be detected to age-related macular degeneration or diagnosing age-related macular degeneration. The age-related macular degeneration is diagnosed by detecting the expression and / or methylation of the CRX gene in blood or serum, the operation feasibility is high, the sampling is convenient, and the accuracy is high.

Description

technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to a method for detecting peripheral blood CRX gene and diagnosing age-related macular degeneration. Background technique [0002] Age-related macular degeneration (AMD) is the leading cause of blindness in patients over the age of 55; by 2040, it will affect about 1.7 billion people, of which 1.1 billion are Asians; the abnormal neovascularization produced by age-related macular degeneration can Causes submacular fluid and lipid leakage, fibrous scarring, and ultimately severe visual impairment. The pathogenesis of AMD is related to many factors such as genetic inheritance and environment. CRX gene is an important transcription factor related to the differentiation of retinal photoreceptors, and plays an important role in maintaining normal cone and rod cell functions. CRX gene mutation can cause retinal photoreceptor cell apoptosis, Leber disease and other fundus diseases. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/154C12Q2600/158
Inventor 于复东许迅刘堃沈胤忱郑颖陈翀熊淑毓成璐
Owner 于复东
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com