A method for improving stress tolerance of rice
A stress tolerance and rice technology, applied in the field of improving the stress tolerance of rice, can solve the problems of unacceptable transgenic plant breeding and difficult plant stress tolerance.
Active Publication Date: 2021-12-28
INST OF BOTANY CHINESE ACAD OF SCI
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Problems solved by technology
However, the previous methods generally required the transfer of exogenous genes, and transgenic plants are difficult to accept in breeding, so this also leads to very few genes that can be actually applied to production
However, due to the complexity of plant stress tolerance traits, it is increasingly difficult to improve plant stress tolerance using traditional breeding methods, which requires us to use new technologies and new ideas to achieve efficient, fast, easy to accept, low A new means of genetic engineering of risky plants stress tolerance
Method used
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Embodiment
[0051] Example, Suppression of OsABA8ox1 Gene by CAS9 Technology to Enhance Stress Tolerance of Rice
[0052] 1. Construction of recombinant plasmids
[0053] 1. Synthesize single-stranded DNA molecule I and single-stranded DNA molecule II.
[0054] Single-stranded DNA molecule I: 5'-TGTGTGGCATCCGCTCCTTGCTCGC-3';
[0055] Single-stranded DNA molecule II: 5'-AAACGCGAGCAAGGAGCGGATGCCA-3'.
[0056] 2. Anneal the single-stranded DNA molecule I and the single-stranded DNA molecule II to obtain a double-stranded DNA molecule with sticky ends.
[0057] 3. Take the double-stranded DNA molecule obtained in step 2 and connect it with the CRISPR / Cas vector to obtain the recombinant plasmid pCRISPR-ABA8ox1.
[0058] According to the sequencing results, the structure of the recombinant plasmid pCRISPR-ABA8ox1 is described as follows: it has a DNA molecule shown in sequence 4 of the sequence listing and expresses the sgRNA shown in sequence 5 of the sequence listing. The schematic diagr...
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The invention discloses a method for improving stress tolerance of rice. The invention provides a method for cultivating plants resistant to abiotic stress, comprising the following steps: inhibiting the expression of the abscisic acid 8'-hydroxylase gene in the starting plant, thereby enhancing the stress tolerance of the plant to the abiotic stress. The abiotic stress is low temperature stress and / or salt stress and / or drought stress. The invention has important theoretical significance and practical value for plant breeding aimed at cultivating stress-tolerant plants, especially rice breeding aimed at cultivating stress-tolerant plants.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving stress tolerance of rice. Background technique [0002] Adversity stresses such as soil salinization, drought, and low temperature have become serious problems affecting agricultural production. Using genetic engineering to improve the stress tolerance of crops and improving the adaptability of crops and economic crops to adversity stress is an urgent need to solve in the cultivation of new varieties. Critical and significant issues. In recent years, people have done a lot of research on the mechanism of plant response to adversity stress from the perspectives of physiology, biochemistry, metabolism, ecology, genetics, and evolution, and accumulated a wealth of data. Especially with the development of molecular biology, people can Understanding the mechanism of plant stress tolerance at the molecular level, such as gene composition, expression regu...
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IPC IPC(8): C12N15/82C12N15/53C12N9/02C12N15/113A01H5/00A01H5/10A01H6/46
CPCC12N15/8213C12N15/8273C12N15/113C12N9/0073C12Y114/13093C12N2810/10C12N2310/10C12N2310/20
Inventor 种康唐永严高莹徐云远
Owner INST OF BOTANY CHINESE ACAD OF SCI