Application of NsNHX1 protein and related biomaterials in breeding of reverse-resistant poplar

A protein and plant stress tolerance technology, applied in the biological field, can solve the problems of lack of development and research of antiporter genes
CN110256544AActive Publication Date: 2019-09-20INNER MONGOLIA UNIVERSITY +1
6 Cites 8 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INNER MONGOLIA UNIVERSITY
Publication Date
2019-09-20

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an application of NsNHX1 protein and related biomaterials in breeding of reverse-resistant poplar. In the present invention, the nitraria sibirica Na<+> / H<+> reverse transport protein NsNHX1 is used as a research object, and is transferred into Populus tomentosa 84K to obtain transgenic NsNHX1 Populus tomentosa. Three transformants, N1, N2 and N3, are selected for identification of reverse resistance. The wild-type Populus tomentosa is used as a control to study the salt-tolerance and antioxidant capacity of transgenic NsNHX1 populus tomentosa under adversity stress. The results show that under the conditions of adversity stress treatment, the survival rate, biomass, leaf chlorophyll content, water content, plant height and antioxidant capacity of transgenic NsNHX1 are significantly higher than those of wild type populus tomentosa. The application indicates that NsNHX1 can significantly improve the reverse resistance and antioxidant capacity of the transgenic poplar, and NsNHX1 can be used as a salt tolerance gene to breed the reverse-resistant poplar varieties.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to the application of NsNHX1 protein and related biological materials in cultivating stress-tolerant poplars. Background technique

[0002] Plant roots are in direct contact with Na in the soil + organs, so the Na in the root + Na + Concentration of compartmentalization is an important mechanism in determining plant salt tolerance. Na on the tonoplast + / H + The antiporter (NHX1) transports Na in the cytoplasm + Transport against the concentration gradient to compartmentalize the vacuole; Na on the plasma membrane + / H + The antiporter protein (SOS1) can transport Na in the cytoplasm + High Na + Environment. So far, the developed and applied Na + / H + Most of the antiporter genes come from Arabidopsis, tomato and other sweet soil plants, but for woody plants, especially for halophytic woody plants Na + / H + The development and research of antiporter genes are r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More