Application of NsNHX1 protein and related biomaterials in breeding of reverse-resistant poplar
A protein and plant stress tolerance technology, applied in the biological field, can solve the problems of lack of development and research of antiporter genes
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[0113] Example 1, the acquisition of NsNHX1 Populus tomentosa and its stress tolerance analysis
[0114] 1. Acquisition of NsNHX1 Populus tomentosa
[0115] 1. Construction of recombinant vector
[0116] (1) Amplification of the target gene
[0117] According to Siberian Nitraria + / H + A pair of primers (NHX-pBI-F: 5'-GC TCTAGA ATGGATCAATTAAGTT-3', 5' includes XbaI recognition sequence; NHX-pBI-R: 5'-C GAGCTC TCACTGCCATTGGGGGAT-3', including the SacI recognition sequence at the 5' end), using the RNA of Nitraria sibirica Pall as a template, using NHX-pBI-F and NHX-pBI-R primers, using TaKaRa RNA PCR Kit (AMV) Ver. The 3.0 kit amplifies the NsNHX1 ORF region to obtain a PCR product. The reaction conditions are: 94°C for 3min; 94°C for 30s, 52°C for 30s, 72°C for 1min, 35cycles; 72°C for 5min. The electrophoresis results of the PCR products were as follows: figure 1 shown.
[0118] (2) The target gene is connected to the expression vector
[0119] The binary expres...
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