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Preparation method of dried porphyridium powder

A technology of Porphyridium algae dry algae powder and Porphyridium algae liquid, applied in the field of marine microorganisms, can solve the problems of uneven combination of algae cells and flocculants, easy agglomeration, low efficiency, etc., to solve the problem of uneven combination, The effect of bright colors, reducing centrifugal force and centrifuging time

Pending Publication Date: 2020-08-14
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there are many technical solutions for adding cationic flocculants to collect microalgae, the main method is to add flocculant powder directly to the algae liquid. Uneven combination, low efficiency, large loss

Method used

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  • Preparation method of dried porphyridium powder
  • Preparation method of dried porphyridium powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Mix the flocculant cationic etherified starch with water, heat at 50°C for 30 minutes, and then cool to room temperature to obtain a flocculant solution with a concentration of 2wt‰;

[0028] (2) The above-mentioned flocculant solution and Porphyridium purpureum CoE1 (in the stable growth phase) are slowly stirred and mixed at room temperature at a volume ratio of 0.5:1 at a speed of 20 rpm (stirring speed is such that no naked eyes will be produced in the liquid). Visible bubbles), so that the cells of Porphyridium coccus and the flocculant are fully flocculated, and then left to stand until solid-liquid separation;

[0029] (3) discard the supernatant of the material obtained in step (2), then remove the remaining moisture by centrifuging at 2000rpm for 10min to obtain a precipitate;

[0030] (4) Mix the above precipitate with water, centrifuge at 2000rpm for 10min and wash 3 times to wash away excess flocculant and unflocculated cells, and collect the algae sludg...

Embodiment 2

[0036] (1) Mix the flocculant cationic etherified starch with water, heat at 70°C for 30 minutes, and then cool to room temperature to obtain a flocculant solution with a concentration of 4wt‰;

[0037] (2) The above-mentioned flocculant solution and Porphyridium purpureum CoE1 (in the stable growth phase) are slowly stirred and mixed at a volume ratio of 1:1 at a speed of 30 rpm (the stirring speed is such that no visible bubbles will be produced in the liquid. ) to fully flocculate the cells of Porphyridium algae and the flocculant, and then leave it to solid-liquid separation;

[0038] (3) Discard the supernatant of the material obtained in step (2), then remove the remaining moisture by centrifuging at 4000rpm for 5min to obtain a precipitate;

[0039] (4) Mix the above precipitate with water, centrifuge at 4000rpm for 5min and wash 3 times to wash away excess flocculant and unflocculated cells, and collect the algae sludge;

[0040] (5) The above-mentioned algae mud is v...

Embodiment 3

[0045](1) Mix the flocculant cationic etherified starch with water, heat at 100°C for 30 minutes, and then cool to room temperature to obtain a flocculant solution with a concentration of 10wt‰;

[0046] (2) The above-mentioned flocculant solution and Porphyridium purpureum CoE1 (in the stable growth phase) are slowly stirred and mixed at a volume ratio of 2:1 at a speed of 40 rpm (the stirring speed is such that no visible bubbles will be produced in the liquid. ) to fully flocculate the cells of Porphyridium algae and the flocculant, and then leave it to solid-liquid separation;

[0047] (3) Discard the supernatant of the material obtained in step (2), then remove the remaining moisture by centrifuging at 8000rpm for 1min to obtain a precipitate;

[0048] (4) Mix the above precipitate with water, centrifuge at 8000rpm for 1min and wash 3 times to wash away excess flocculant and unflocculated cells, and collect the algae sludge;

[0049] (5) The above-mentioned algae mud is ...

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Abstract

The invention discloses a preparation method of dried porphyridium powder. A flocculating agent solution is prepared and the use concentration of the flocculating agent solution is optimized; an algaeliquid into the flocculating agent solution; and slow stirring is carried out. The effect of rapid flocculation can be achieved in a very short time, and the problem of non-uniform combination is well solved. The preparation method has a good flocculation effect, flocculated algae can be directly separated, and the centrifugal force and the centrifugal time are greatly reduced. The obtained algaehas a bright color and well retains the color of the algae, cost for industrial production is saved, and efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of marine microorganisms, and in particular relates to a method for preparing dried porphyridium algae powder. Background technique [0002] Porphyridium purpureum is a relatively primitive unicellular red alga first discovered by Naegeli in 1849. It belongs to Rhodophyta, Protoflorideophyceae, Porphyridiales, Porphyridiaceae, Porphyridium. Among many microalgae strains, Porphyridium has the characteristics of high biomass yield and high value-added products, and Porphyridium is distributed in seawater, freshwater, salt water and wet land, and has strong salt resistance. Porphyridium algae cells can synthesize a variety of biologically active substances during the growth process, and have broad application prospects in industries such as medicine, cosmetics, food, health care products, textiles, printing and dyeing, metallurgy and petroleum. [0003] The individual cells of Porphyridium algae are tiny, wit...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 曾宪海徐远超吴升山李闯彭志清唐兴孙勇林鹿
Owner XIAMEN UNIV
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