Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Indirect ELISA detection kit and detection method of mycoplasma gallisepticum antibody

A technology of Mycoplasma gallisepticum and a kit can be applied in the biological field and can solve the problems of high detection cost and narrow promotion scope.

Inactive Publication Date: 2020-08-14
CHINA AGRI UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, detection kits from companies such as IDEXX are usually used for MG serological monitoring, which is expensive and limited in scope of promotion.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Indirect ELISA detection kit and detection method of mycoplasma gallisepticum antibody
  • Indirect ELISA detection kit and detection method of mycoplasma gallisepticum antibody
  • Indirect ELISA detection kit and detection method of mycoplasma gallisepticum antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1, utilize PVPA recombinant protein to detect Mycoplasma gallisepticum antibody

[0088] 1. Preparation of PVPA recombinant protein

[0089] 1.1 Gene preparation

[0090] Utilize the primer pair that Mqpvpa-F and Mqpvpa-R form to carry out PCR amplification to the genomic DNA of MG strain, Mqpvpa-F and Mqpvpa-R are respectively two single-stranded DNAs shown in sequence 3 and 4 in the sequence list, to The obtained PCR product is sequenced, and the result shows that its sequence is sequence 1 in the sequence listing, and the 9th-1133rd position of sequence 1 is the PVPA gene sequence of the MG strain, and the 35th-408th position of sequence 2 in the coding sequence list is shown of PVPA protein.

[0091] 1.2 Construction of recombinant vector

[0092] The PCR product obtained in step 1.1 was digested with restriction endonucleases BamHI-HF and XhoI to obtain a PCR product digestion product; the PET-28a (+) vector was digested with restriction endonucleases...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses an indirect ELISA detection kit and a detection method of a mycoplasma gallisepticum antibody. The indirect ELISA detection kit of the mycoplasma gallisepticum antibody comprises a PVPA protein, 2.5% skim milk powder, 5% BSA, a coating stabilizer, MG positive serum, MG negative serum, 0.01 M PBS (pH 7.2-7.4), horse radish peroxidase labeled rabbit anti-chicken IgY, a microwell plate, a washing solution, a chromogenic substrate and a stop solution. The PVPA protein is a protein of which the amino acid sequence is 35th-408th sites of a sequence 2 or a protein of the sequence 2. The kit can be used for detecting whether serum of an animal to be detected contains the mycoplasma gallisepticum antibody or not and judging whether the animal to be detected is infected withmycoplasma gallisepticum or not, a reliable detection method is provided for identifying MG infection, and scientific guidance is provided for MG epidemic prevention and control.

Description

technical field [0001] The invention relates to an indirect ELISA detection kit and a detection method for mycoplasma gallisepticum antibody in the field of biotechnology. Background technique [0002] Mycoplasma gallisepticum (MG) is the pathogen of chronic respiratory disease (CRD) in chickens. It can cause damage to the respiratory tract, air sacs and infraorbital sinuses of chickens. The egg production rate of laying hens decreases, the quality of broilers decreases, and the abandonment rate increases. It is a serious hazard to the poultry industry. one of the infectious diseases. MG can be transmitted vertically through eggs, resulting in decreased hatching rate and fertilization rate, increased chick mortality and growth and development disorders, and making the offspring of breeders become MG-positive flocks. This is an important reason why the disease is very common. It is very difficult to control and purify the pathogen. Therefore, it is of great significance to d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/543
CPCG01N33/54306G01N33/56933G01N33/6854G01N2469/20
Inventor 吴文学王丰乔西兰
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com