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A detection kit for forest encephalitis virus igm antibody and its application

A forest encephalitis virus, detection kit technology, applied in biological testing, measuring devices, immunoassays, etc., can solve problems such as decreased detection accuracy, and achieve the effect of improving stability

Active Publication Date: 2021-11-23
长春生物制品研究所有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the applicant found in follow-up research that even if the kit disclosed in the above patent application is stored in a low temperature environment, there is still a defect that the detection accuracy is significantly reduced

Method used

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  • A detection kit for forest encephalitis virus igm antibody and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) According to the description of Examples 1 and 2 in the specification of CN109613249A, a specific monoclonal antibody against forest encephalitis virus was prepared. After testing, the neutralizing titer of the monoclonal antibody was 1:32 times.

[0020] (2) Prepare the enzyme-labeled reagent according to the formula shown in the table below, and test its stability during long-term storage.

[0021] group Formula (monoclonal antibody concentration after dilution is 1mg / ml Control group 1 PBS containing 5% bovine serum albumin as diluent, Control group 2 Diluent in PBS containing 3% sucrose + 8% glycerol Control group 3 Diluent in PBS containing 0.1% gelatin + 8% glycerol Control group 4 Diluent in PBS containing 0.1% EDTA+1.5% sorbitol Experimental group 1 Diluent in PBS containing 0.1% vitamin C + 1% ethylene glycol Experimental group 2 Diluent in PBS containing 0.15% vitamin C + 0.5% ethylene glycol Experim...

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Abstract

The present invention relates to a forest encephalitis virus IgM antibody detection kit and application thereof, said kit includes enzyme-labeled forest encephalitis virus Mori Zhang strain E protein monoclonal antibody reagent, said enzyme-labeled forest encephalitis virus Mori The diluent containing vitamin C and ethylene glycol is used as a stabilizer in the monoclonal antibody reagent of Zhang Zhu E protein. The kit of the present invention can maintain stability and effectiveness for a long time, and can maintain a potency of more than 60% (preferably more than 70%) even if it is stored in the dark at a temperature of 0-4°C for more than 200 days, which is of great importance for clinical use. significance.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a medical detection kit, more specifically to a forest encephalitis virus IgM antibody detection kit and its application. Background technique [0002] Forest encephalitis virus, also known as tick-borne encephalitis virus (TBEV), belongs to the Flavivirus genus. Forest encephalitis virus is a spherical particle, and its genome is a single-stranded positive-strand RNA with a length of about 11kb, encoding three structures in total Proteins are capsid protein (C), membrane protein (M), envelope protein (E) and 7 non-structural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5), among the 3 structural proteins, The E protein is the most important structural protein of the virus, which contains a variety of T cell epitopes and B cell epitopes, which determine the tissue tropism of the virus, mediate the binding of the virus to the cell membrane receptor, and induce a protective imm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/577G01N33/569G01N33/543G01N33/531
CPCG01N33/531G01N33/54393G01N33/56983G01N33/577G01N33/581G01N33/6854G01N2333/185G01N2469/20
Inventor 常军亮邹勇孙宏亮曹玉峰张秀霞唐剑光吴月韩慧利李雨桐严永男
Owner 长春生物制品研究所有限责任公司