Miniature zooming living brain imaging system

Abstract

The invention discloses a miniature zooming living brain imaging system comprising a micro microscope, an implantation lens and a focusing base; the implantation lens is implanted into a target area of the brain of an experimental animal through an operation. The focusing base is fixed on the surface of the head of the experimental animal; the implantation lens and the focusing base are mounted, the focusing base can be mounted on the implantation lens or detached from the implantation lens, the micro microscope is mounted on the focusing base or detached from the focusing base, and the micromicroscope is mounted on the implantation lens in a matched mode through the focusing base and carries out focusing adjustment. The miniature zooming living brain imaging system can be disassembled and assembled on the focusing base at any time in any physiological state of the experimental animal, the experimental animal can wear the system for a long time, and living body intracerebral imaging can be carried out on the experimental animal under various physiological activity conditions.

Description

technical field [0001] The present invention relates to a method and system for fluorescent microscopic imaging, and more particularly, relates to a miniature zoom living brain imaging system. Background technique [0002] One of the central questions in neuroscience currently being studied is how neural activity in living organisms creates distinct brain functions and the behaviors that result from these functions. Therefore, the neural activity must be recorded during the behavior of the organism to determine the causal relationship between the neural activity and the biological behavior process. [0003] In the field of basic medicine, researchers generally use model animals such as mice and rats, combined with a series of imaging techniques. The commonly used imaging method in basic medical research at this stage is microscopic fluorescence imaging of tissue slices, that is, the imaging results are obtained under a wide-field fluorescence microscope or a fluorescence co...

AI Technical Summary

Problems solved by technology

However, generally speaking, fluorescence microscopes are huge in size, and it is difficult to follow live animals in real-time imaging in a free-moving state; there are also some optical fiber invasive transmission methods for fluorescence excitation and collection, but the collection field of view is small and the point is fixed. The shortcomings of low resolution and low signal-to-noise ratio; in addition, there are wearable miniature two-photon or wide-field fluorescence microscopes. Although they can achieve good imaging results, on the one hand, the depth of focus is not deep, and they cannot Internal control changes the focus position. On the other hand, due to design reasons, there are also disadvantages such as inflexible use and cumbersome operation.

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