Pharmaceutical composition for treating dentin allergy and preparation method and application thereof

A composition and drug technology, applied in the direction of drug combination, pharmaceutical formula, allergic diseases, etc., can solve the problems that natural NCPs are not easy to obtain and have limited wide application, so as to promote deposition and directional growth, ensure blockage, and improve the effect of treatment

Pending Publication Date: 2020-08-18
苏州知会智能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the extraction and purification of natural NCPs are not readily available, their widespread application in oral clinics is still limited.

Method used

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  • Pharmaceutical composition for treating dentin allergy and preparation method and application thereof
  • Pharmaceutical composition for treating dentin allergy and preparation method and application thereof
  • Pharmaceutical composition for treating dentin allergy and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation and experiment of embodiment 1 amphiphilic oligopeptide

[0035] 1. Preparation of amphiphilic oligopeptides

[0036] The amphipathic oligopeptide consists of a hydrophilic peptide sequence ()ASN-ASN-CYS-CYS-CYS-CYS-ARG-ARG-GLU-SER ((p)) mimicking CEMP1 and a hydrophobic Tail (C 16 h 31 COOH) composition. Oligopeptides were synthesized by solid-phase peptide synthesis, stepwise from the C-terminus to the N-terminus using an automated synthesizer.

[0037] The oligopeptide powder was dissolved in deionized water, and the self-assembly of the amphiphilic oligopeptide was initiated with 1 M calcium chloride solution.

[0038] Afterwards, the suspension was ultrasonically mixed to promote the diffusion of calcium ions, and then incubated at 4°C to obtain self-assembled oligopeptides.

[0039] The secondary structure of self-assembled oligopeptides was characterized by far ultraviolet circular dichroism chromatography. The self-assembled oligopeptides were ...

Embodiment 2

[0051] Embodiment 2 results and characterization

[0052] 1. Preparation of amphiphilic oligopeptides

[0053] Loss of enamel, superficial dentin, and cementum on the dentin surface is probably the most common cause of dentin tubule exposure. Therefore, the reconstruction of dental hard tissue is an important research field for the treatment of dentin sensitivity. The formation of inorganic minerals in dental tissue, such as nucleation, phase transition, and orientation growth, requires the regulation of organic biomolecules. In the present invention, the oligopeptide was designed based on the principle of the functional amino acid sequence of CEMP1 responsible for cementum mineralization and amphiphile. Amphiphilic oligopeptides can self-assemble into supramolecular structures through hydrophobic interactions. Therefore, exposure of bioactive peptides to an aqueous environment modulates mineralization.

[0054] In the present invention, Ca 2+ Used to trigger self-assembl...

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Abstract

The invention discloses a pharmaceutical composition for treating dentin allergy and a preparation method and application thereof. The invention belongs to the technical field of dental sclerotin regeneration. The pharmaceutical composition comprises a pharmacodynamically effective amount of amphiphilic oligopeptide, and the amphiphilic oligopeptide comprises a hydrophilic peptide and a hydrophobic tail with an amino acid sequence represented by SEQ ID No.1, and pharmaceutically acceptable pharmaceutical excipients, the oligopeptide simulating CEMP1 is used for regenerating enamel-like tissueson the surface of dentin and sealing dentinal tubules, so that the treatment of dentin allergy is improved, the self-assembled oligopeptide has very strong binding capacity to acid etching dentin, and deposition and directional growth of apatite microcrystals are promoted, so that an enamel-like structure is constructed on the surface of the dentin, then, the regenerated microcrystals are deeplydeposited in the dentin tubules, and effective blockage of the dentin tubules is ensured, therefore, the CEMP1-simulating amphiphilic oligopeptide has great application potential in the treatment on dentin hypersensitivity in the future.

Description

technical field [0001] The invention relates to the technical field of cementum regeneration, in particular to a pharmaceutical composition for treating dentin hypersensitivity, a preparation method and application thereof. Background technique [0002] Dentin hypersensitivity (Dentine hypersensitivity, DH) is a common and frequently-occurring disease in stomatology, and the prevalence of DH in the general population is about 15-25%. DH can be defined as brief, intense pain or discomfort in response to stimuli in the oral environment, such as temperature, chemicals, osmotic pressure, and mechanical effects. The development of DH is thought to be due to the exposure of deep dentin due to the loss of enamel, superficial dentin and cementum. Dentin tubules are covered and entangled by enamel at the crown, superficial dentin, and cementum at the root, protecting the deep dentin from external stimuli. Patients with DH often experience pain or discomfort when consuming stimulati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61P1/02A61P37/08
CPCA61K38/08A61P1/02A61P37/08
Inventor 孙卫斌王晴晴
Owner 苏州知会智能科技有限公司
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