Method for culturing microalgae by using benzoic acid

A benzoic acid and microalgae technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of limiting the growth of microalgae cells, reducing the overall biological yield, etc., so as to improve the composition of oil and improve the yield. , Improve the effect of oil conversion rate

Active Publication Date: 2020-08-18
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, many scholars use extreme abiotic stress conditions such as low temperature, high light, oligotrophy, and high salt to induce the growth of microalgae. Althoug

Method used

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  • Method for culturing microalgae by using benzoic acid
  • Method for culturing microalgae by using benzoic acid
  • Method for culturing microalgae by using benzoic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] autotrophic model

[0025] Inoculate 10% chlorella into the basic BG11 medium for cultivation, and after 12 days of cultivation, obtain chlorella at the initial stage of the growth adjustment period;

[0026] The chlorella at the initial stage of the growth adjustment period was inoculated in the medium that added benzoic acid (BA) to the basic BG11 medium, wherein the concentration of benzoic acid was 500 μg / L. After mixing, the chlorella cells were mixed by 10 % volume fraction was inoculated into the above-mentioned BA-containing medium, and the initial concentration was adjusted to OD680 of 0.5. After mixing, it was placed in a photoreactor with a temperature of 20°C, a rotation speed of 150rpm, a light intensity of 4000Lux, and a light / dark ratio It is 16h / 8h, and it will be harvested on the 12th day after multiplication.

Embodiment 2

[0028] In the glucose-containing medium, inoculate 10% chlorella for cultivation, and after culturing for 8 days, obtain chlorella at the initial stage of the growth adjustment period, wherein the glucose-containing medium contains 10 g of glucose per liter of BG11 medium;

[0029] Inoculate the chlorella at the initial stage of the growth adjustment period into the medium containing benzoic acid (BA) in the basic BG11 medium containing glucose, wherein the concentration of benzoic acid is 500 μg / L. After mixing, the chlorella Cells were inoculated into the above-mentioned BA-containing medium at a volume fraction of 10%, and the initial concentration was adjusted to OD680 of 0.5. After mixing, they were placed in a photoreactor, the light mode was turned off, and the whole was covered with tinfoil. The temperature is room temperature (about 20° C.), the rotation speed is 150 rpm, and the harvest is performed on the 8th day after multiplication.

Embodiment 3

[0031] The condition parameters are the same as in Example 1, except that in this example, 10% Scenedesmus will be inoculated for cultivation.

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Abstract

The invention belongs to the technical field of microalgae culture, and particularly relates to a method for culturing microalgae by using benzoic acid. The method comprises the following steps of: adding benzoic acid at the initial stage of the growth regulation period of microalgae, and continuing culture, wherein the addition amount of benzoic acid is 50-2,000 microgram/L. By adopting the method provided by the invention, the biomass of the microalgae is increased by 6.67%-27%, the grease content is improved by 13.65%-45.6%, the content of chlorophyll a is increased by 4.55%-60%, the content of chlorophyll b is increased by 7.14%-71%, especially the content of carotenoid is increased by 31.9%-153%, the protein yield is increased by 26.2%-28.6%, and the saccharide yield is increased by 11.8%-18.2%.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a method for cultivating microalgae with benzoic acid. Background technique [0002] Microalgae can not only be used as raw materials for biofuels, but also produce a variety of high-value intracellular products, which is a high-yield and sustainable bioenergy and biomass raw material. Moreover, the extracts in the algae cells have outstanding biological activity, and have the functions of anti-oxidation, anti-inflammation and reduction of cardiovascular diseases. [0003] At present, many scholars use extreme abiotic stress conditions such as low temperature, high light, oligotrophy, and high salt to induce the growth of microalgae. Although these methods are beneficial to the increase of the content of target components in cells, they also limit the growth of microalgae cells, thereby reducing the the overall biomass. It can be seen that the low bio...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/20C12N1/38C12P7/64C12R1/89C12R1/01
CPCC12N1/12C12N1/20C12N1/38C12P7/6463
Inventor 周丹丹赵振豪付亮崔晓春刘洋
Owner NORTHEAST NORMAL UNIVERSITY
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