Method for separating gelsemium elegans alkaloid monomers from gelsemium elegans alkaloids by combining high-speed counter-current chromatography with preparative liquid chromatography

Abstract

The invention discloses a method for separating gelsemium elegans alkaloid monomers from gelsemium elegans alkaloids by combining high-speed counter-current chromatography with preparative liquid chromatography. The method comprises the following steps: determining target gelsemium elegans alkaloid monomer components from a gelsemium elegans extract by adopting high performance liquid chromatography, sequentially carrying out primary separation by virtue of high-speed counter-current chromatography and secondary separation by virtue of preparative liquid chromatography, and respectively collecting each target gelsemium elegans alkaloid monomer component. According to the method, a plurality of chromatographic instruments are combined to separate the gelsemium elegans monomers, so that themethod has the advantages of large preparation amount, short period, high purity and the like, eight gelsemium elegans alkaloid monomers can be separated at the same time, and the method has controllability, automatic productivity and wide application prospect.

Description

technical field [0001] The present invention relates to a method for separating Gelzia alkaloid monomers from Gelzia alkaloids, in particular to a method for separating and preparing Gelzia alkaloids monomers from Gelzia alkaloids by using high-speed countercurrent chromatography and preparative liquid chromatography. A body method belongs to the technical field of separation of active ingredients of natural medicines. Background technique [0002] Gelsemium elegans, also known as heartbroken grass, big tea medicine, cannon leaf, yellow flower bitter vine, yellow mangcai, etc., is an evergreen woody vine of the genus Gelsemium elegans, produced in Jiangxi, Fujian, Taiwan, Hunan, In Guangdong, Hainan, Guangxi, Guizhou, Yunnan and other provinces and regions, it has the effects of reducing swelling and pain, removing poison and killing insects. It is often used as a traditional Chinese veterinary herbal medicine. It has the effect of repelling insects on pigs, cattle, and shee...

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Problems solved by technology

[0005] In view of the defects existing in the prior art, the object of the present invention is to provide a method for rapidly and efficiently separating and preparing various Gelcinnas alkaloid monomers from Gelkins alkaloids by using a combination of high-speed countercurrent chromatography and preparative liquid chromatography. method, this method overcomes the defect that the general method in the prior art is difficult to simultaneously separate a variety of gelzia alkaloid monomers

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