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Lactobacillus casei capable of relieving osteoporosis and application of lactobacillus casei

A Lactobacillus casei, osteoporosis technology, applied in the field of microorganisms, can solve the problems of increasing the risk of venous thromboembolism and stroke, and achieve the effect of high safety

Active Publication Date: 2020-08-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Selective estrogen receptor modulator raloxifene increases risk of venous thromboembolism and stroke

Method used

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  • Lactobacillus casei capable of relieving osteoporosis and application of lactobacillus casei
  • Lactobacillus casei capable of relieving osteoporosis and application of lactobacillus casei
  • Lactobacillus casei capable of relieving osteoporosis and application of lactobacillus casei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1: the acquisition of Lactobacillus casei

[0077] Specific steps are as follows:

[0078] Taking fresh feces from healthy people in Ningde, Fujian as samples, draw 0.5mL sample and add it to 5mL MRS liquid medium, culture at 37°C for 18-24h, and enrich to obtain the enriched sample; draw 0.5mL enriched The pooled sample was added to 4.5mL sterile saline to obtain 10 -1 Diluent, then draw 0.5mL 10 -1 Diluted solution in 4.5mL normal saline to obtain 10 -2 Diluent, according to this operation, successively get 10 -3 , 10 -4 , 10 -5 , 10 -6 Dilution; draw 100 μL of gradient dilution and spread it on the MRS solid medium, 10 -4 , 10 -5 , 10 -6 1 plate for each gradient, cultivated at 37°C for 48 hours to obtain colonies; select the colonies with typical characteristics of Lactobacillus casei on the MRS solid medium according to the shape, size, edge, and transparency of the colonies, and use an inoculation loop to pick the colonies on the MRS solid cult...

Embodiment 2

[0084] Example 2: Effects of Lactobacillus casei on bone mineral density, bone volume fraction and trabecular bone thickness in ovariectomized rats

[0085] Specific steps are as follows:

[0086] Take 20 SPF grade female SD rats with a body weight of 250±20g, and divide them into 4 groups at random, 5 rats in each group. CCFM1127 group and HN13 group gavaged with Lactobacillus casei HN13 suspension.

[0087] The experiment lasted for 9 weeks: the first week was the adaptation period for the rats. During the adaptation period, the rats were fed in an environment with a temperature of 22±2°C, a humidity of 40-70%, and a 12-hour alternation of day and night. The feed used was purchased from Suzhou Shuangshi Rat breeding compound feed from Experimental Animal Feed Technology Co., Ltd. was used for the experiment after a week of normal diet adaptation.

[0088] After the end of the first week, the rats in the model control group, CCFM1127 group and HN13 group were anesthetized b...

Embodiment 3

[0093] Embodiment 3: Effect of Lactobacillus casei on immune factor levels in ovariectomized rat serum

[0094] Specific steps are as follows:

[0095] Take 20 SPF grade female SD rats with a body weight of 250±20g, and divide them into 4 groups at random, 5 rats in each group. CCFM1127 group and HN13 group gavaged with Lactobacillus casei HN13 suspension.

[0096] The experiment lasted for 9 weeks: the first week was the adaptation period for the rats. During the adaptation period, the rats were fed in an environment with a temperature of 22±2°C, a humidity of 40-70%, and a 12-hour alternation of day and night. The feed used was purchased from Suzhou Shuangshi Rat breeding compound feed from Experimental Animal Feed Technology Co., Ltd. was used for the experiment after a week of normal diet adaptation.

[0097] After the end of the first week, the rats in the model control group, CCFM1127 group and HN13 group were anesthetized by intraperitoneal injection of 10% (v / v) chlo...

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Abstract

The invention discloses lactobacillus casei capable of relieving osteoporosis and an application of the lactobacillus casei, which belongs to the technical field of microorganisms and medicines. The invention provides a lactobacillus casei CCFM1127, and the lactobacillus casei CCFM1127 can alleviate osteoporosis, which is specifically embodied in that the bone mineral density level of ovariectomized rats is significantly improved; the bone volume fraction level of ovariectomized rats is obviously improved; the bone trabecula thickness level of ovariectomized rats is obviously improved; the content of IL-1 beta in ovariectomized rat serum is obviously reduced; the content of TNF-alpha in ovariectomized rat serum is obviously reduced; the content of IL-10 in serum of ovariectomized rats is remarkably increased; the content of endotoxin in serum of ovariectomized rats is remarkably reduced, so that the lactobacillus casei CCFM1127 has a huge application prospect in preparation of productsfor preventing and / or treating osteoporosis.

Description

technical field [0001] The invention relates to a strain of Lactobacillus casei which can relieve osteoporosis and application thereof, and belongs to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Osteoporosis (osteoporosis) is a common bone disease, it is caused by a variety of reasons, with the increasing aging of society, more and more people suffer from osteoporosis. The clinical classification of osteoporosis is: primary, secondary and idiopathic. Among them, primary osteoporosis is a physiological degenerative disease that occurs with age, mainly manifested as postmenopausal osteoporosis and senile osteoporosis; secondary osteoporosis is caused by Metabolic diseases or long-term use of certain drugs are induced by some factors, such as hyperthyroidism or hypothyroidism, acromegaly, diabetes, etc.; idiopathic osteoporosis is more common in adolescents, often accompanied by genetic family history, female More t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P19/10A61P1/10A61P1/14A61P1/04A23L33/135C12R1/245
CPCA61K35/747A61P19/10A61P1/10A61P1/14A61P1/04A23L33/135A23V2002/00C12R2001/245C12N1/205A23V2400/125A23V2200/306A23V2200/32
Inventor 翟齐啸陈卫彭江田丰伟于雷雷赵建新张灏
Owner JIANGNAN UNIV
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