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Excrement metabolite and detection system for detecting active pulmonary tuberculosis

A technology of active pulmonary tuberculosis and detection system, which is applied in the field of biomarkers, can solve the problems of low sensitivity and insufficient resolution, and achieve the effect of good repeatability, small injection volume and no sample

Active Publication Date: 2020-08-21
广东省结核病控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest disadvantage of NMR technology is low sensitivity, insufficient resolution, and high-abundance analytes often mask low-abundance analytes

Method used

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  • Excrement metabolite and detection system for detecting active pulmonary tuberculosis
  • Excrement metabolite and detection system for detecting active pulmonary tuberculosis
  • Excrement metabolite and detection system for detecting active pulmonary tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A detection system for detecting active pulmonary tuberculosis, comprising parameter acquisition equipment and a data processing device with the following data processing functions:

[0040] Analyze the results of the test patient's feces detected by LC-MS, and output the conclusion according to the following standard: if one or more of the 13 differential metabolites in the test patient's feces, their relative expression levels are all >2X Un group average value (i.e. 2 times the mean value of the uninfected Mtb healthy group) and the relative expression levels are >2X the mean value of the LTBI group (i.e. 2 times the mean value of the uninfected Mtb healthy group), then the patient to be tested is or is a candidate for activity tuberculosis patients; the above 13 differential metabolites include 1-Naphthylamine 1-naphthylamine, Bilirubin bilirubin, delta-Hydroxylysineδhydroxylysine, Docosanamide, Dodemorph, Himbacine, Indolelactic acid, N-Cyclohexylformamide N-cycloh...

Embodiment 2

[0047] Example 2 A method for judging active pulmonary tuberculosis based on feces LC-MS technology

[0048] 1. Inclusion and grouping of research objects

[0049] The included population is from Shenzhen Chronic Hospital, aged 18-60 years, with regular work, rest and diet, and no alcoholism or smoking. The specific groups are as follows:

[0050] (1) The healthy group (45 people) who were not infected with tuberculosis, that is, those who were not infected with Mtb. This group of people had no symptoms and signs of tuberculosis and related mycobacterial diseases, and the gamma interferon release test was negative (excluding the pre-allergy stage; immunization System interference: application of glucocorticoids and other immunosuppressants or malnutrition, measles, pertussis, etc.; immunocompromised: severe tuberculosis, various critically ill patients, lymphocyte system immune deficiency, etc.) (people without PPD, use tuberculosis antibody IgMIgG (Colloidal gold method) in...

Embodiment 3

[0092] Example 3 The verification of the method for distinguishing the patient to be tested as an active pulmonary tuberculosis patient

[0093] The feces of 4 patients with active pulmonary tuberculosis (ATB), 4 cases of Mtb latent healthy people and 6 cases of healthy people without Mtb infection were routinely collected (the feces came from Shenzhen Chronic Hospital, all patients were confirmed by clinical diagnosis, and patients and volunteers All informed), according to the method of judging active pulmonary tuberculosis patients based on feces LC-MS metabolomics analysis technology in Example 1, the relative expression levels of 13 differential metabolites were respectively obtained. 13 differential metabolites including 1-Naphthylamine 1-naphthylamine, Bilirubin bilirubin, delta-Hydroxylysineδhydroxylysine, Docosanamide, Dodemorph, Himbacine, Indolelactic acid , N-Cyclohexylformamide N-cyclohexylformamide, Neotame N-[N-(3,3-dimethylbutyl)-L-α-aspartyl]-L-phenylalanine-1...

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Abstract

The invention discloses an excrement metabolite and detection system for detecting active pulmonary tuberculosis. The excrement metabolite is selected from 13 kinds of differential metabolites. The detection system is based on an excrement LC-MS metabonomics technology, LC-MS detection is carried out on excrement of a to-be-detected patient, the relative expression quantities of 13 different metabolites are obtained respectively, and then whether the to-be-detected patient is an active pulmonary tuberculosis patient or not is judged according to the relative expression quantities of the metabolites. Compared with the traditional method, the method provided by the invention has the advantages of non-invasion, simple sample treatment, no damage to the sample, small sample injection amount, good repeatability, low cost and the like.

Description

technical field [0001] The present invention relates to the technical field of biomarkers, more specifically, to feces metabolites and a detection system for detecting active pulmonary tuberculosis. Background technique [0002] Tuberculosis (TB) is a widespread and in many cases fatal chronic infectious disease caused by Mycobacterium tuberculosis (Mtb) infection. most. Although the incidence of tuberculosis has been slowly declining in recent years, there were still about 10 million new cases and about 1.7 million deaths worldwide in 2018. Tuberculosis has become a global problem threatening human health, and has become the leading cause of death in some developing countries and regions, especially in areas with a high incidence of AIDS. [0003] Early diagnosis of tuberculosis and timely anti-tuberculosis treatment are of great significance to effectively control the progression of tuberculosis and the spread of Mycobacterium tuberculosis. Currently, methods for diagno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/7266
Inventor 魏文静周琳陈亮陈燕梅巫株华张晨晨郭卉欣黄新春孙琦卓文基董文雅
Owner 广东省结核病控制中心
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