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Novel coronavirus total antibody detection test strip, detection device comprising novel coronavirus total antibody detection test strip and preparation method and application thereof

A coronavirus and detection test strip technology, applied in the field of immune detection, can solve the problems of shortage of diagnostic reagents, threats to the personal health of operators, hidden dangers and other problems, and achieve the effect of fast detection speed, high safety and high safety.

Pending Publication Date: 2020-08-21
北京康思润业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now there are some new coronavirus antibody detection reagents on the market. Most of the reagents directly expose the sample or the dilution containing the sample. There are certain safety hazards in the detection process, which may threaten the personal health of operators.
Therefore, the production of relatively safe diagnostic reagents is still in short supply

Method used

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  • Novel coronavirus total antibody detection test strip, detection device comprising novel coronavirus total antibody detection test strip and preparation method and application thereof
  • Novel coronavirus total antibody detection test strip, detection device comprising novel coronavirus total antibody detection test strip and preparation method and application thereof
  • Novel coronavirus total antibody detection test strip, detection device comprising novel coronavirus total antibody detection test strip and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Preparation of novel coronavirus total antibody detection test strip

[0055] 1. Preparation of colloidal gold solution

[0056] Under the magnetic stirring condition of keeping 200r / min, add 500ml ultrapure water in clean 1L flask, heat 3 minutes with 200 ℃; 3 minutes); then add 2.8ml of 12wt% trisodium citrate solution, and continue heating at 200°C for 4 minutes (the color of the solution changes to yellow-white-black-purple-burgundy); after cooling to room temperature, add purified water Dilute to 500ml and store in a clean brown reagent bottle at 2°C to 8°C.

[0057] 2. Colloidal gold labeling

[0058] Add 100ml of the colloidal gold solution prepared above to two 250ml beakers respectively, add 750μl of 0.2M K 2 CO 3 solution, mix well; then add the 2019-nCoV NF protein (purchased from the Institute of Microbiology, Chinese Academy of Sciences) and the mouse IgG polyclonal antibody (purchased from Luoyang Bio-Tong Experimental Materials Center) to be...

Embodiment 2

[0071] Example 2 Assembly of novel coronavirus total antibody detection device

[0072] The new coronavirus total antibody detection test strip prepared in Example 1 can be inserted into a safety tube to prepare a new coronavirus total antibody detection device; or inserted into a safety tube for detection during detection.

[0073] The safety tube (such as figure 2 (shown) may include: a tube body 10 and a plug 20 buckled on the tube body 10 , the tube body 10 is selected from the prior art, and is generally a test tube. The size of the test tube is set as follows: the length is 8cm-15cm, and the diameter is 1cm-2cm.

Embodiment 3

[0074] Example 3 detection

[0075] 3.1 The safety tube is placed vertically

[0076] Open the tube stopper 20, add sample buffer solution into the tube body 10, the volume of the sample buffer solution is 160 μl; add the blood sample into the above tube body, the sample volume is 20 μl, use a sampler to blow or shake it manually, so that the test paper One end of the water-absorbing pad 4 of the strip is immersed in the liquid, but note that the liquid level of the liquid cannot exceed the MAX line of the test strip (the MAX line is 15mm away from the detection line); cover the tube plug tightly, place it vertically, and react for 10-15min; The formula of the sample buffer is as follows: 50 mM PB, 0.9 wt% NaCl, 0.25 wt% Tween-20, 0.1 wt% Casein. Among them, PB is phosphate buffer solution; NaCl is sodium chloride; Tween-20 is Tween 20; Casein is casein.

[0077] After the reaction time is over, if bands appear on both the detection line 31 and the quality control line 32 of...

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Abstract

The invention relates to a novel coronavirus total antibody detection test strip, a detection device comprising the novel coronavirus total antibody detection test strip and a preparation method and application of the novel coronavirus total antibody detection test strip. The detection test strip comprises a sample pad; a marker pad coupled to the sample pad; a coating pad connected to the markerpad and a water absorption pad; a detection line and a quality control line are sequentially arranged on the coating pad, the detection line is coated with a strain of novel coronavirus virus antigen;and the quality control line is coated with a quality control antibody. According to the method, the professional requirement limitation of PCR on instrument operation, personnel, environment and thelike can be improved, the epidemic first-line suspected case screening can be accelerated, confirmed personnel isolation can be rapidly carried out, and the social panic emotion can be effectively reduced.

Description

technical field [0001] The invention relates to a novel coronavirus total antibody detection test strip, a detection device including the same, a preparation method and application thereof, and belongs to the technical field of immunodetection. Background technique [0002] Recently, the COVID-19 epidemic has become increasingly serious around the world, which has had a great impact on social life and economic development. Although new coronavirus detection reagents emerge in endlessly, due to the high infectivity of the new coronavirus, not only medical staff who directly contact patients need to be carefully protected, but also relevant personnel who diagnose and test patients and contact patient samples also need to pay attention to safety issues . Now there are some new coronavirus antibody detection reagents on the market. Most of the reagents directly expose the sample or the diluent containing the sample. There are certain safety hazards in the detection process, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558
CPCG01N33/6854G01N33/587G01N33/56983G01N33/558G01N2333/165G01N2469/20
Inventor 张军李海燕
Owner 北京康思润业生物技术有限公司