Method for inducing embryogenic callus of test tube corms of Jiangxi Yanshan Colocasia esulenla Schott

An embryogenic callus and red taro technology, applied in the field of callus culture, can solve the problems of low callus induction rate and regeneration rate, difficulty in callus induction and the like

Active Publication Date: 2020-08-25
SHANGRAO NORMAL UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]In the prior art, although there are many studies on the tissue culture of Jiangxi Qianshan red bud taro, the research on the use of explants to induce ca

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing embryogenic callus of test tube corms of Jiangxi Yanshan Colocasia esulenla Schott

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) One-time pretreatment: select the test tube bulbs induced by Jiangxi Yanshan red bud taro as explants, and place them in MS containing 0.05g / L chitooligosaccharide and 0.3-0.5g / L graphene quantum dots under sterile conditions. Soak in the culture medium for 48h;

[0035] (2) Secondary pretreatment: under aseptic conditions, place the test tube corms induced by Jiangxi Qianshan red bud taro after pretreatment in step (1) in a place containing 0.05g / L chitosan oligosaccharide and 0.3-0.5g / Soak the MS culture solution of L carbon nanotubes for 48 hours;

[0036] (3) Embedding: under aseptic conditions, cut the Jiangxi Yanshan red bud taro test tube bulbs with a size of about (1-1.5) cm × (0.5-1) cm and a thickness of 0.2-0.3 mm. Thin slices are mixed evenly with the calcium ion-free improved MS culture solution containing 3% (W / V) sodium alginate and 0.2mol / L sucrose, and the mixed solution is dripped into L graphene quantum dots, 0.03-0.05g / L carbon nanotubes, 0.1m...

Embodiment 2

[0041] (1) One-time pretreatment: select the test tube bulbs induced by Jiangxi Yanshan red bud taro as explants, and place them in a medium containing 0.05g / L chitosan oligosaccharide and 0.3-0.5g / L graphene quantum dots under sterile conditions. Soak in MS culture solution for 48h;

[0042] (2) Secondary pretreatment: under aseptic conditions, place the test tube corms induced by Jiangxi Qianshan red bud taro after pretreatment in step (1) in a place containing 0.05g / L chitosan oligosaccharide and 0.3-0.5g / Soak the MS culture solution of L carbon nanotubes for 48 hours;

[0043] (3) Embedding: under aseptic conditions, cut the Jiangxi Yanshan red bud taro test tube bulbs with a size of about (1-1.5) cm × (0.5-1) cm and a thickness of 0.2-0.3 mm. Thin slices are mixed evenly with the calcium ion-free improved MS culture solution containing 3% (W / V) sodium alginate and 0.2mol / L sucrose, and the mixed solution is dripped into L graphene quantum dots, 0.03-0.05g / L carbon nano...

Embodiment 3

[0048] (1) One-time pretreatment: select the test tube bulbs induced by Jiangxi Yanshan red bud taro as explants, place them in MS culture medium containing 0.05g / L chitosan oligosaccharide and 0.3-0.5g / L graphene quantum dots at room temperature Soak in medium for 48h;

[0049] (2) Secondary pretreatment: at room temperature, place the test tube corm induced by Jiangxi Qianshan red bud taro pretreated in step (1) in a place containing 0.05g / L chitosan oligosaccharide and 0.3-0.5g / L carbon Soak nanotubes in MS culture solution for 48 hours;

[0050] (3) Embedding: Cut the Jiangxi Yanshan red bud taro test tube corm with a size of about (1-1.5) cm × (0.5-1) cm and a thickness of 0.2-0.3 mm into thin slices at room temperature. The calcium ion-free modified MS culture solution containing 3% (W / V) sodium alginate and 0.2mol / L sucrose is mixed evenly, and the mixed solution is added dropwise into Quantum dots, 0.03-0.05g / L carbon nanotubes, 0.1mol / LCaCl 2 and 0.2mol / L sucrose M...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for inducing embryogenic callus of test tube corms of Jiangxi Yanshan Colocasia esulenla Schott, and belongs to the technical field of callus culture. The method comprises the following steps: taking test tube corms induced byJiangxi Yanshan Colocasia esulenla Schott as explants, slicing the test tube corms subjected to two-time pretreatment, embedding the sliced test tube corms to obtain test tube corm slice embedded beads, and then inoculating the embedded beads to solid culture media with different nutritional ingredients for culture so that the embryogeniccallus of the test tube corm slices of Jiangxi Yanshan Colocasia esulenla Schott can be induced. By the callus induction method disclosed by the invention, the induction rate of the embryogenic callusis as high as 95% or above, and the regeneration rate of the embryogenic callus can be 100%.

Description

technical field [0001] The invention relates to the technical field of callus culture, in particular to a method for inducing bulbous embryogenic callus of Jiangxi Yanshan red bud taro test tube. Background technique [0002] Red bud taro is a traditional superior product in Qianshan County, Jiangxi Province, with a long history of planting. Jiangxi Yanshan red bud taro has bright skin color, red terminal buds, tender meat, easy to boil but not mushy, rich nutrition, contains a variety of trace elements, is a treasure among taro, and has always been a hot commodity in domestic and foreign markets. It is the flagship product of Qianshan County. In the past ten years, this advantageous industry has developed rapidly and has become the leading industry for farmers in Yanshan County to increase their income and become rich. Qianshan County has also become the largest red bud taro production base in Jiangxi Province. In March 2007, it was approved by China Green Food Committee a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 尹明华杨玉琪洪森荣
Owner SHANGRAO NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap