A preparation method of a drug-loaded ultrasound contrast agent using macrophages as a carrier

Abstract

The invention discloses a preparation method of a drug-loaded ultrasonic contrast agent using macrophages as a carrier, comprising: a. preparation of cationic microbubbles; b. preparation of drug-loaded cationic microbubbles; c. drug-loaded cationic fluorocarbon droplets d, preparation of β‑glucan-modified drug-loaded cationic fluorocarbon droplets; e, preparation of macrophage ultrasonic diagnostic agent. The present invention uses low-temperature phase-change nano-droplets to mark cells, which can solve the defects of poor stability, short lifespan and low safety in vivo of cells marked by ultrasonic contrast agents in the past. The limitation of low cell labeling rate of ultrasound contrast agents in the past can be solved by preparing ultrasound diagnostic agents specifically targeting macrophages. The "Trojan horse" effect can be used to target the target area, increase the imaging effect of the target organ and the concentration of the drug, thereby increasing the efficacy of the drug and reducing the side effects. It can be applied to various related diseases involving macrophages. Combine ultrasound imaging with drug therapy to realize the integration of diagnosis and treatment.

Description

technical field [0001] The invention relates to the technical field of ultrasound contrast and biological materials, in particular to a preparation method of a drug-loaded ultrasound contrast agent using macrophages as a carrier. Background technique [0002] In recent years, many studies have used the biological functions of cells to use living cells as biological carriers for the study of disease mechanisms and their diagnosis and treatment. Modified stem cells and T cells are often used to diagnose diseases in combination with photoacoustic, ultrasound, MRI, CT and other imaging modalities. In terms of ultrasound imaging, some studies have reported that mesoporous silicon is used as an ultrasound contrast agent to label bone marrow stem cells for imaging, but it has the defect of poor biodegradability. It is also useful to label cells with traditional ultrasound contrast agent microbubbles (Microbubbles). For example, the patent application number CN201610017740.5 disclo...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a preparation method of a drug-loaded ultrasonic contrast agent with macrophages as a carrier, to solve the problem of poor biodegradability, low safety in vivo, poor stability, low labeling rate and imaging problems of existing ultrasonic contrast agents. Problems with little effect

[0008] In order to realize the preparation method of the above-mentioned drug-loaded ultrasound contrast agent with macrophages as the carrier and solve the problems of poor biodegradability, low safety in vivo, poor stability, low labeling rate and inconspicuous imaging effect of the existing ultrasound contrast agent, this paper The invention provides the following technical solutions:

Images