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Method for raising astragalus seedlings with high flavone content

A technology of high flavonoid content and astragalus, which is applied to the field of astragalus seedlings with high flavonoid content and plant seedlings, can solve the problems of planting degradation, shortening of seedling raising period, imperfect management, etc., and achieves accelerated growth rate, promotion of rapid accumulation, and improvement of survival rate. Effect

Inactive Publication Date: 2020-08-28
内蒙古自治区生物技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, the scale of industrialized planting of Astragalus membranaceus has been expanding year by year, but the scale and technology of Astragalus membranaceus seedlings are seriously lagging behind, and they are still stuck in the traditional extensive original seedlings and planting stage. This backward planting method relies more on natural cultivation and imperfect management, resulting in Degradation, unstable medicinal ingredients, large differences, and low yield; in order to solve the above problems, the soilless cultivation technology of Astragalus came into being, especially the development of water culture seedling technology has expanded the scale of Astragalus seedlings, increased the survival rate, and shortened the seedling cycle. shortened, has good application development prospects
[0005] However, in the process of artificial cultivation of Astragalus membranaceus, the germination process of Astragalus membranaceus seeds, as well as the hydroponic nutrient solution, and the development of hydroponic conditions are not perfect, resulting in low germination rate of Astragalus membranaceus seeds, unstable survival rate of Astragalus membranaceus seedlings, and long growth cycle. Long, and the active ingredient is unstable

Method used

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  • Method for raising astragalus seedlings with high flavone content
  • Method for raising astragalus seedlings with high flavone content

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Experimental program
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Effect test

Embodiment 1

[0026] A method for cultivating Astragalus seedlings with high flavonoid content, comprising the following steps:

[0027] (1) Take an appropriate amount of Astragalus mongolica seeds, add 60% ethanol solution 3 times the weight of the seeds, ultrasonic treatment time 60s; wash 5 times with water; soak in ice water for 4 hours, take out and soak in water at 70°C for 2 hours Then, add 6% sodium hypochlorite solution 3 times of seed weight and soak for 15 minutes, wash with water 5 times and drain;

[0028] (2) Put the Astragalus membranaceus seeds in step (1) into MS medium for cultivation, the cultivation temperature is 20° C., humidity 60%, cultivate for 12 days to germinate, and replace the MS medium once every 4 days;

[0029] The MS medium contains the following components by weight per liter: KNO3 6mM, Ca(NO3)2.4H2O 6mM, MgSO4.7H2O 2.5mM, KH2PO4 1.2mM, H3BO3 48μM, MnCl2.4H2O 10μM, ZnSO4.7H2O0.8μM, CuSO4·5H2O 0.4μM, Na2MoO4·2H2O 0.3μM, EDTA-Fe-Na 60μM;

[0030] (3) Add t...

Embodiment 2

[0035] A method for cultivating Astragalus seedlings with high flavonoid content, comprising the following steps:

[0036] (1) Take an appropriate amount of Astragalus membranaceus seeds, add 75% ethanol solution with a concentration 3 times the weight of the seeds, after ultrasonic treatment for 40 seconds; wash with water for 5 times; soak in ice water for 4 hours, take out and soak in water at 90°C 1h; then, add 4 times the weight of 1% mercuric chloride solution to soak for 15min, wash with water 5 times and drain;

[0037] (2) Put the Astragalus membranaceus seeds in the step (1) into the MS medium for cultivation, the cultivation temperature is 20° C., the humidity is 60%, and they are cultivated for 10 days to germinate, and the MS medium is replaced once every 2 days;

[0038]The MS medium contains the following components per liter by weight: KNO3 5mM, Ca(NO3)2.4H2O 4mM, MgSO4.7H2O 2mM, KH2PO4 1mM, H3BO3 45μM, MnCl2.4H2O 9μM, ZnSO4.7H2O0.7μM, CuSO4. 5H2O 0.2μM, Na2Mo...

Embodiment 3

[0044] A method for cultivating Astragalus seedlings with high flavonoid content, comprising the following steps:

[0045] (1) Take an appropriate amount of Astragalus mongolica seeds, add a 60% ethanol solution that is 4 times the weight of the seeds, and ultrasonicate for 60 seconds; wash with water for 4 times; soak in ice water for 3 hours, take it out and soak in water at 80°C for 2 hours Then, add 1% mercuric chloride solution 3 times of seed weight and soak for 12 minutes, wash with water 4 times and drain;

[0046] (2) Put the Astragalus membranaceus seeds in the step (1) into the MS medium for cultivation, the cultivation temperature is 25° C., the humidity is 60%, and they are cultivated for 12 days to germinate, and the MS medium is replaced once every 3 days;

[0047] The MS medium contains the following components by weight per liter: KNO3 6mM, Ca(NO3)2.4H2O 5mM, MgSO4.7H2O 1.8mM, KH2PO4 1.1mM, H3BO3 45μM, MnCl2.4H2O 8μM, ZnSO4.7H2O0.6μM, CuSO4·5H2O 0.2μM, Na2MoO...

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Abstract

The invention provides a method for raising astragalus seedlings with high flavone content. The method comprises the steps of germination of astragalus seeds and culture of astragalus seedlings. By optimizing the composition and proportion of an astragalus water culture nutrient solution, a nutrient solution suitable for nutrient elements required by growth of the astragalus seedlings is screened,and rapid growth of the astragalus seedlings is promoted; furthermore, by optimizing the technological conditions of astragalus seedling raising, especially pretreatment of the astragalus seeds and application of blue light and yellow light in the seedling raising process, the germination rate of the astragalus seeds is increased, the survival rate of the raised seedlings is increased, the flavone content in the astragalus seedlings is increased, and the method has high market application value.

Description

technical field [0001] The invention relates to a method for growing seedlings of plants, in particular to a method for raising seedlings of astragalus with high flavonoid content, and belongs to the technical field of plant cultivation. Background technique [0002] Astragalus membranaceus (Astragalus membranaeus (Fisch.) Bge) and Mongolian Astragalus membranaceus (Astragalus membranaceus Bge.var.mongholicus (Bge.) hsiao) are the dried roots of Leguminosae plants. The main pharmacological effects of Astragalus include invigorating qi and strengthening the surface, diuresis, detoxification and expulsion of pus, and promoting muscle growth. It is mainly used to treat shortness of breath, collapse, palpitations, spontaneous sweating, physical weakness and edema, chronic nephritis, dehydration, chronic diarrhea, qi deficiency, and insufficient qi and blood. , Sinking of Qi, has antibacterial and anti-inflammatory, antihypertensive and diuretic, detoxifying and choleretic effect...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/04A01C1/00A01C1/08A61L2/04C05G5/20C05G1/00
CPCA01C1/00A01C1/08A01G7/045A01G31/00A61L2/04C05B7/00C05G5/20C05C1/00C05C5/02C05C5/04C05D5/00C05D9/02C05F11/00C05F11/10
Inventor 张秀娟白朕卿焦巍
Owner 内蒙古自治区生物技术研究院
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