A blood detection method and blood analysis system

A technology for blood testing and blood samples, applied in the field of blood analysis systems and the optical detection of platelets, can solve the problems of inability to distinguish between red blood cell fragments and platelets, and achieve the effect of reducing testing costs and simplifying blood testing.

Active Publication Date: 2021-10-12
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to be performed in a separate test channel, and this stain still cannot effectively distinguish between red blood cell fragments and platelets

Method used

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  • A blood detection method and blood analysis system
  • A blood detection method and blood analysis system
  • A blood detection method and blood analysis system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0358] Embodiment 1 Deep hemolysis treatment detects platelets

[0359] Prepare the hemolytic agent of the present invention according to the following formula.

[0360]

[0361] Add 20 microliters of fresh blood samples to 1 mL of the prepared solution above, incubate at 45°C for 60 seconds to prepare the samples to be tested, and then measure them with a flow cytometer (BriCyte E6). Set the gain to 500 for data collection, collect the side scattered light with a measurement angle of 90 degrees to obtain the side scattered light intensity information of the particles in the sample; and collect the forward scattered light signal of 0 degrees. Scatter distribution of platelets and white blood cells Figure 10 shown, from Figure 10 It can be seen that red blood cell fragments, platelets, and white blood cells are highly differentiated, and these three groups of particles can be clearly distinguished, and platelets and white blood cells can be effectively classified and cou...

Embodiment 2

[0363] Example 2 Contrasting the performance of deep hemolysis treatment and conventional hemolysis treatment on platelets

[0364] Prepare the detection reagent of the present invention according to the following formula.

[0365]

[0366] 20 microliters of fresh blood samples were added to 1 mL of the solution prepared according to the above formula, incubated at 45°C for 60 seconds, and then detected by a flow cytometer (BriCyte E6). Set the excitation wavelength to 488nm, the gain to 500, collect the 0° forward scattered light intensity and 90° side scattered light intensity information, and obtain the two-dimensional cell scatter diagram as shown in Figure 11 Shown in A.

[0367] As a comparison, 20 microliters of fresh blood from the same blood sample was added to 1 mL of LD hemolysis agent (which contains conventional hemolysis agent) matched with Mindray blood analyzer BC-6800, incubated at 45°C for 60 seconds, and then analyzed by flow cytometry. (Mindray BriCyt...

Embodiment 3

[0369] Example 3 Deep Hemolysis and Adding Nucleic Acid Dyes Counting Platelets and White Blood Cells by Side Light Scattering and Fluorescent Signals

[0370] Prepare the detection reagent of the present invention according to the following formula.

[0371]

[0372] 20 microliters of fresh blood samples were added to 1 mL of the solution prepared according to the above formula, incubated at 45°C for 60 seconds, and then detected by a flow cytometer (BriCyte E6). Set the excitation wavelength to 488nm, the gain to 500, collect the 90-degree side fluorescence intensity and the 0-degree forward scattered light intensity information, and obtain the two-dimensional cell scatter diagram as shown in Figure 12 As shown in A.

[0373] It can be seen from the figure that in the state of hemolysis, the addition of nucleic acid dyes can effectively distinguish and display platelets and RET in the direction of fluorescence. By dividing the proportion of PLT scattered points, and ac...

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Abstract

The invention discloses a blood detection method, comprising: processing a blood sample with a first reagent to obtain a test sample, the first reagent includes a hemolytic agent, and the hemolytic agent lyses red blood cells in the blood sample into its Fragments whose light scattering properties are significantly different from platelets; make the particles in the sample to be tested pass through the detection area of ​​the optical detection system one by one, and obtain the optical information of the sample to be tested; and obtain according to at least two of the optical information Optical information of platelets. The method of the present invention obtains platelet count by lysing red blood cells in a blood sample, and can simultaneously distinguish white blood cell subgroups.

Description

technical field [0001] The invention relates to blood detection, in particular to an optical detection method of platelets and a blood analysis system thereof. Background technique [0002] Human blood contains various cells such as red blood cells, white blood cells, and platelets, among which platelets are non-nucleated cells with a diameter of 2-3 microns. The blood of normal people contains 150,000 to 350,000 platelets per microliter. [0003] As we all know, one of the commonly used assay methods for platelets is the electrical impedance method. The method is to pass a sample containing blood cells through a small hole with two electrodes. When blood cells (such as platelets) pass through, the resistance changes, thereby generating a resistance pulse, and then the detected pulse is drawn into a histogram for analysis. . In normal blood, platelets are the smallest, white blood cells are the largest, and red blood cells are in the middle. The detected pulse intensity c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/49G01N15/14G01N35/00
CPCG01N15/1459G01N2015/0084G01N2015/1006G01N33/49G01N35/00G01N15/1429G01N33/5094
Inventor 陈庚文张子千叶燚李朝阳
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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